4EN6

Crystal structure of HA70 (HA3) subcomponent of Clostridium botulinum type C progenitor toxin in complex with alpha 2-3-sialyllactose

Summary for 4EN6

• Entry DOI: 10.2210/pdb4en6/pdb
• Related: 2ZOE 2ZS6 4EN7 4EN8 4EN9
• Descriptor: Hemagglutinin components HA-22/23/53, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, (4R)-2-METHYLPENTANE-2,4-DIOL, ... (5 entities in total)
• Functional Keywords: carbohydrate/sugar binding, sugar binding protein
• Biological source: Clostridium botulinum; More
• Total number of polymer chains: 2
• Total formula weight: 74608.81

Authors

Yamashita, S.,Yoshida, H.,Tonozuka, T.,Nishikawa, A.,Kamitori, S. (deposition date: 2012-04-12, release date: 2012-06-06, Last modification date: 2023-11-08)

Primary citation

Yamashita, S.,Yoshida, H.,Uchiyama, N.,Nakakita, Y.,Nakakita, S.,Tonozuka, T.,Oguma, K.,Nishikawa, A.,Kamitori, S.
Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides
Febs Lett., 586:2404-2410, 2012

PubMed Abstract: Clostridium botulinum produces the botulinum neurotoxin, forming a large complex as progenitor toxins in association with non-toxic non-hemagglutinin and/or several different hemagglutinin (HA) subcomponents, HA33, HA17 and HA70, which bind to carbohydrate of glycoproteins from epithelial cells in the infection process. To elucidate the carbohydrate recognition mechanism of HA70, X-ray structures of HA70 from type C toxin (HA70/C) in complexes with sialylated oligosaccharides were determined, and a binding assay by the glycoconjugate microarray was performed. These results suggested that HA70/C can recognize both α2-3- and α2-6-sialylated oligosaccharides, and that it has a higher affinity for α2-3-sialylated oligosaccharides.

PubMed: 22684008
DOI: 10.1016/j.febslet.2012.05.055

Experimental method

X-RAY DIFFRACTION (2.56 Å)