4EGW

The structure of the soluble domain of CorA from Methanocaldococcus jannaschii

4EGW の概要

• エントリーDOI: 10.2210/pdb4egw/pdb
• 分子名称: Magnesium transport protein CorA, HEXANE-1,6-DIOL, 1,4-BUTANEDIOL, ... (6 entities in total)
• 機能のキーワード: magnesium transporter, magnesium binding, cora, metal transport
• 由来する生物種: Methanocaldococcus jannaschii
• 細胞内の位置: Cell membrane; Multi-pass membrane protein (By similarity): Q58439
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68700.81

構造登録者

Guskov, A.,Nordin, N.,Reynaud, A.,Engman, H.,Lundback, A.-K.,Jong, A.J.O.,Cornvik, T.,Phua, T.,Eshaghi, S. (登録日: 2012-04-02, 公開日: 2012-10-31, 最終更新日: 2024-03-20)

主引用文献

Guskov, A.,Nordin, N.,Reynaud, A.,Engman, H.,Lundback, A.K.,Jong, A.J.,Cornvik, T.,Phua, T.,Eshaghi, S.
Structural insights into the mechanisms of Mg2+ uptake, transport, and gating by CorA
Proc.Natl.Acad.Sci.USA, 109:18459-18464, 2012

PubMed Abstract: Despite the importance of Mg(2+) for numerous cellular activities, the mechanisms underlying its import and homeostasis are poorly understood. The CorA family is ubiquitous and is primarily responsible for Mg(2+) transport. However, the key questions-such as, the ion selectivity, the transport pathway, and the gating mechanism-have remained unanswered for this protein family. We present a 3.2 Å resolution structure of the archaeal CorA from Methanocaldococcus jannaschii, which is a unique complete structure of a CorA protein and reveals the organization of the selectivity filter, which is composed of the signature motif of this family. The structure reveals that polar residues facing the channel coordinate a partially hydrated Mg(2+) during the transport. Based on these findings, we propose a unique gating mechanism involving a helical turn upon the binding of Mg(2+) to the regulatory intracellular binding sites, and thus converting a polar ion passage into a narrow hydrophobic pore. Because the amino acids involved in the uptake, transport, and gating are all conserved within the entire CorA family, we believe this mechanism is general for the whole family including the eukaryotic homologs.

PubMed: 23091000
DOI: 10.1073/pnas.1210076109

実験手法

X-RAY DIFFRACTION (2.5 Å)