4EGG

Computationally Designed Self-assembling tetrahedron protein, T310

4EGG の概要

• エントリーDOI: 10.2210/pdb4egg/pdb
• 関連するPDBエントリー: 3V4E 4DCL
• 分子名称: Putative acetyltransferase SACOL2570, GLYCEROL (3 entities in total)
• 機能のキーワード: self assembling tetrahedron design, transferase
• 由来する生物種: Staphylococcus aureus subsp. aureus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 138875.23

構造登録者

Sawaya, M.R.,King, N.P.,Sheffler, W.,Baker, D.,Yeates, T.O. (登録日: 2012-03-30, 公開日: 2012-05-30, 最終更新日: 2023-09-13)

主引用文献

King, N.P.,Sheffler, W.,Sawaya, M.R.,Vollmar, B.S.,Sumida, J.P.,Andre, I.,Gonen, T.,Yeates, T.O.,Baker, D.
Computational design of self-assembling protein nanomaterials with atomic level accuracy.
Science, 336:1171-1174, 2012

PubMed Abstract: We describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to design a wide variety of self-assembling protein nanomaterials.

PubMed: 22654060
DOI: 10.1126/science.1219364

実験手法

X-RAY DIFFRACTION (2.21 Å)