4EF3

Multicopper Oxidase CueO (Citrate buffer)

4EF3 の概要

• エントリーDOI: 10.2210/pdb4ef3/pdb
• 分子名称: Blue copper oxidase CueO, COPPER (II) ION, HYDROXIDE ION, ... (6 entities in total)
• 機能のキーワード: multicopper oxidase, metal binding protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P36649
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54019.65

構造登録者

Komori, H.,Kataoka, K.,Sakurai, T.,Higuchi, Y. (登録日: 2012-03-29, 公開日: 2013-05-22, 最終更新日: 2024-03-20)

主引用文献

Komori, H.,Kataoka, K.,Tanaka, S.,Matsuda, N.,Higuchi, Y.,Sakurai, T.
Exogenous acetate ion reaches the type II copper centre in CueO through the water-excretion channel and potentially affects the enzymatic activity.
Acta Crystallogr.,Sect.F, 72:558-563, 2016

PubMed Abstract: The acetate-bound form of the type II copper was found in the X-ray structure of the multicopper oxidase CueO crystallized in acetate buffer in addition to the conventional OH(-)-bound form as the major resting form. The acetate ion was retained bound to the type II copper even after prolonged exposure of a CueO crystal to X-ray radiation, which led to the stepwise reduction of the Cu centres. However, in this study, when CueO was crystallized in citrate buffer the OH(-)-bound form was present exclusively. This fact shows that an exogenous acetate ion reaches the type II Cu centre through the water channel constructed between domains 1 and 3 in the CueO molecule. It was also found that the enzymatic activity of CueO is enhanced in the presence of acetate ions in the solvent water.

PubMed: 27380373
DOI: 10.1107/S2053230X16009237

実験手法

X-RAY DIFFRACTION (1.9 Å)