4E9L

FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections

「3TKV」から置き換えられました

4E9L の概要

• エントリーDOI: 10.2210/pdb4e9l/pdb
• 分子名称: Attaching and effacing protein, pathogenesis factor (2 entities in total)
• 機能のキーワード: invasin-like, bacterial immunoglobulin, pathogenic, vaccine, cell adhesion
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42623.85

構造登録者

Spraggon, G.,Nesta, B.,Alteri, C.,Gomes Moriel, D.,Rosini, R.,Veggi, D.,Smith, S.,Bertoldi, I.,Pastorello, I.,Ferlenghi, I.,Fontana, M.R.,Frankel, G.,Mobley, H.L.T.,Rappuoli, R.,Pizza, M.,Serino, L.,Soriana, M. (登録日: 2012-03-21, 公開日: 2012-04-25, 最終更新日: 2023-09-13)

主引用文献

Nesta, B.,Spraggon, G.,Alteri, C.,Moriel, D.G.,Rosini, R.,Veggi, D.,Smith, S.,Bertoldi, I.,Pastorello, I.,Ferlenghi, I.,Fontana, M.R.,Frankel, G.,Mobley, H.L.,Rappuoli, R.,Pizza, M.,Serino, L.,Soriani, M.
FdeC, a novel broadly conserved Escherichia coli adhesin eliciting protection against urinary tract infections.
MBio, 3:-, 2012

PubMed Abstract: The increasing antibiotic resistance of pathogenic Escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine Escherichia coli antigens that protect mice from sepsis. In this study, we characterized one of them, ECOK1_0290, named FdeC (factor adherence E. coli) for its ability to mediate E. coli adhesion to mammalian cells and extracellular matrix. This adhesive propensity was consistent with the X-ray structure of one of the FdeC domains that shows a striking structural homology to Yersinia pseudotuberculosis invasin and enteropathogenic E. coli intimin. Confocal imaging analysis revealed that expression of FdeC on the bacterial surface is triggered by interaction of E. coli with host cells. This phenotype was also observed in bladder tissue sections derived from mice infected with an extraintestinal strain. Indeed, we observed that FdeC contributes to colonization of the bladder and kidney, with the wild-type strain outcompeting the fdeC mutant in cochallenge experiments. Finally, intranasal mucosal immunization with recombinant FdeC significantly reduced kidney colonization in mice challenged transurethrally with uropathogenic E. coli, supporting a role for FdeC in urinary tract infections.

PubMed: 22496310
DOI: 10.1128/mBio.00010-12

実験手法

X-RAY DIFFRACTION (1.9 Å)