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4E6H

CRYSTAL STRUCTURE OF THE HAT domain of k. lactis RNA14

4E6H の概要
エントリーDOI10.2210/pdb4e6h/pdb
関連するPDBエントリー2OND 2OOE 4E85 4EBA
分子名称mRNA 3'-end-processing protein RNA14 (2 entities in total)
機能のキーワードhat domain, heat repeat, clp1, pcf11, structural protein
由来する生物種Kluyveromyces lactis (Yeast)
細胞内の位置Nucleus : Q6CII8
タンパク質・核酸の鎖数1
化学式量合計79714.48
構造登録者
Paulson, A.R.,Tong, L. (登録日: 2012-03-15, 公開日: 2012-05-02, 最終更新日: 2024-02-28)
主引用文献Paulson, A.R.,Tong, L.
Crystal structure of the Rna14-Rna15 complex.
Rna, 18:1154-1162, 2012
Cited by
PubMed Abstract: A large protein machinery is required for 3'-end processing of mRNA precursors in eukaryotes. Cleavage factor IA (CF IA), a complex in the 3'-end processing machinery in yeast, contains four subunits, Rna14, Rna15, Clp1, and Pcf11. Rna14 has a HAT (half a TPR) domain at the N terminus and a region at the C terminus that mediates interactions with Rna15. Rna15 contains a RNA recognition module (RRM) at the N terminus, followed by a hinge region. These two proteins are homologs of CstF-77 and CstF-64 in the cleavage stimulation factor (CstF) of the mammalian 3'-end processing machinery. We report the first crystal structure of Rna14 in complex with the hinge region of Rna15, and the structures of the HAT domain of Rna14 alone in two different crystal forms. The complex of the C-terminal region of Rna14 with the hinge region of Rna15 does not have strong interactions with the HAT domain of Rna14, and this complex is likely to function independently of the HAT domain. Like CstF-77, the HAT domain of Rna14 is also a tightly associated dimer with a highly elongated shape. However, there are large variations in the organization of this dimer among the Rna14 structures, and there are also significant structural differences to CstF-77. These observations suggest that the HAT domain and especially its dimer may have some inherent conformational variability.
PubMed: 22513198
DOI: 10.1261/rna.032524.112
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.3 Å)
構造検証レポート
Validation report summary of 4e6h
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-09に公開中

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