4E1S

X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7

4E1S の概要

• エントリーDOI: 10.2210/pdb4e1s/pdb
• 関連するPDBエントリー: 4E1T
• 分子名称: Intimin, CHLORIDE ION, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (5 entities in total)
• 機能のキーワード: outer membrane beta barrel, adhesin, translocated intimin receptor, cell adhesion
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell outer membrane; Single-pass membrane protein (By similarity): P43261
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32457.07

構造登録者

Fairman, J.W.,Dautin, N.,Wojtowicz, D.,Wei, L.,Noinaj, N.,Barnard, T.J.,Udho, E.,Finkelstein, A.,Przytycka, T.M.,Cherezov, V.,Buchanan, S.K. (登録日: 2012-03-07, 公開日: 2012-06-13, 最終更新日: 2024-02-28)

主引用文献

Fairman, J.W.,Dautin, N.,Wojtowicz, D.,Liu, W.,Noinaj, N.,Barnard, T.J.,Udho, E.,Przytycka, T.M.,Cherezov, V.,Buchanan, S.K.
Crystal Structures of the Outer Membrane Domain of Intimin and Invasin from Enterohemorrhagic E. coli and Enteropathogenic Y. pseudotuberculosis.
Structure, 20:1233-1243, 2012

PubMed Abstract: Intimins and invasins are virulence factors produced by pathogenic Gram-negative bacteria. They contain C-terminal extracellular passenger domains that are involved in adhesion to host cells and N-terminal β domains that are embedded in the outer membrane. Here, we identify the domain boundaries of an E. coli intimin β domain and use this information to solve its structure and the β domain structure of a Y. pseudotuberculosis invasin. Both β domain structures crystallized as monomers and reveal that the previous range of residues assigned to the β domain also includes a protease-resistant domain that is part of the passenger. Additionally, we identify 146 nonredundant representative members of the intimin/invasin family based on the boundaries of the highly conserved intimin and invasin β domains. We then use this set of sequences along with our structural data to find and map the evolutionarily constrained residues within the β domain.

PubMed: 22658748
DOI: 10.1016/j.str.2012.04.011

実験手法

X-RAY DIFFRACTION (1.855 Å)