4DYX

Crystal Structure of the Cu-adduct of Human H-Ferritin variant 4His-delta C-star

4DYX の概要

• エントリーDOI: 10.2210/pdb4dyx/pdb
• 関連するPDBエントリー: 2CEI 4DYY 4DYz 4DZ0
• 分子名称: Ferritin heavy chain, COPPER (II) ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: four-helix bundle, oxidoreductase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20466.73

構造登録者

Tezcan, F.A.,Huard, D.J.E. (登録日: 2012-02-29, 公開日: 2013-01-23, 最終更新日: 2023-09-13)

主引用文献

Huard, D.J.,Kane, K.M.,Tezcan, F.A.
Re-engineering protein interfaces yields copper-inducible ferritin cage assembly.
Nat.Chem.Biol., 9:169-176, 2013

PubMed Abstract: The ability to chemically control protein-protein interactions would allow the interrogation of dynamic cellular processes and lead to a better understanding and exploitation of self-assembling protein architectures. Here we introduce a new engineering strategy--reverse metal-templated interface redesign (rMeTIR)--that transforms a natural protein-protein interface into one that only engages in selective response to a metal ion. We have applied rMeTIR to render the self-assembly of the cage-like protein ferritin controllable by divalent copper binding, which has allowed the study of the structure and stability of the isolated ferritin monomer, the demonstration of the primary role of conserved hydrogen-bonding interactions in providing geometric specificity for cage assembly and the uniform chemical modification of the cage interior under physiological conditions. Notably, copper acts as a structural template for ferritin assembly in a manner that is highly reminiscent of RNA sequences that template virus capsid formation.

PubMed: 23340339
DOI: 10.1038/nchembio.1163

実験手法

X-RAY DIFFRACTION (1.85 Å)