4DWW

Crystal Structure of Nattokinase from Bacillus subtilis natto

4DWW の概要

• エントリーDOI: 10.2210/pdb4dww/pdb
• 分子名称: Subtilisin NAT, CALCIUM ION (3 entities in total)
• 機能のキーワード: fibrinolytic activity, hydrolase
• 由来する生物種: Bacillus subtilis subsp. natto
• 細胞内の位置: Secreted: P35835
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27978.95

構造登録者

Chatake, T.,Yanagisawa, Y. (登録日: 2012-02-27, 公開日: 2012-03-14, 最終更新日: 2023-11-08)

主引用文献

Yanagisawa, Y.,Chatake, T.,Chiba-Kamoshida, K.,Naito, S.,Ohsugi, T.,Sumi, H.,Yasuda, I.,Morimoto, Y.
Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase from Bacillus subtilis natto
Acta Crystallogr.,Sect.F, 66:1670-1673, 2010

PubMed Abstract: Nattokinase is a single polypeptide chain composed of 275 amino acids (molecular weight 27,724) which displays strong fibrinolytic activity. Moreover, it can activate other fibrinolytic enzymes such as pro-urokinase and tissue plasminogen activator. In the present study, native nattokinase from Bacillus subtilis natto was purified using gel-filtration chromatography and crystallized to give needle-like crystals which could be used for X-ray diffraction experiments. The crystals belonged to space group C2, with unit-cell parameters a=74.3, b=49.9, c=56.3 Å, β=95.2°. Diffraction images were processed to a resolution of 1.74 Å with an Rmerge of 5.2% (15.3% in the highest resolution shell) and a completeness of 69.8% (30.0% in the highest resolution shell). This study reports the first X-ray diffraction analysis of nattokinase.

PubMed: 21139221
DOI: 10.1107/S1744309110043137

実験手法

X-RAY DIFFRACTION (1.74 Å)