4DWL

Avd molecule from Bordetella bacteriophage DGR

4DWL の概要

• エントリーDOI: 10.2210/pdb4dwl/pdb
• 分子名称: Bbp7, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: pentameric four helix bundle, accessory variability determinant from bordetella bacteriophage diversity generating retroelements, reverse transcriptase, phosphate binding protein, nucleic acid binding protein
• 由来する生物種: Bordetella phage BPP-1
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 75139.18

構造登録者

Ghosh, P.,Al-Ayyoubi, M. (登録日: 2012-02-24, 公開日: 2013-02-27, 最終更新日: 2024-11-06)

主引用文献

Alayyoubi, M.,Guo, H.,Dey, S.,Golnazarian, T.,Brooks, G.A.,Rong, A.,Miller, J.F.,Ghosh, P.
Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.
Structure, 21:266-276, 2013

PubMed Abstract: Diversity-generating retroelements (DGRs) are the only known source of massive protein sequence variation in prokaryotes. These elements transfer coding information from a template region (TR) through an RNA intermediate to a protein-encoding variable region. This retrohoming process is accompanied by unique adenine-specific mutagenesis and, in the prototypical BPP-1 DGR, requires a reverse transcriptase (bRT) and an accessory variability determinant (bAvd) protein. To understand the role of bAvd, we determined its 2.69 Å resolution structure, which revealed a highly positively charged pentameric barrel. In accordance with its charge, bAvd bound both DNA and RNA, albeit without a discernable sequence preference. We found that the coding sequence of bAvd functioned as part of TR but identified means to mutate bAvd without affecting TR. This mutational analysis revealed a strict correspondence between retrohoming and interaction of bAvd with bRT, suggesting that the bRT-bAvd complex is important for DGR retrohoming.

PubMed: 23273427
DOI: 10.1016/j.str.2012.11.016

実験手法

X-RAY DIFFRACTION (2.69 Å)