4DS8

Complex structure of abscisic acid receptor PYL3-(+)-ABA-HAB1 in the presence of Mn2+

Summary for 4DS8

• Entry DOI: 10.2210/pdb4ds8/pdb
• Related: 3KLX 3OJI 4DSB 4DSC
• Descriptor: Abscisic acid receptor PYL3, Protein phosphatase 2C 16, (2Z,4E)-5-[(1S)-1-hydroxy-2,6,6-trimethyl-4-oxocyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid, ... (6 entities in total)
• Functional Keywords: abscisic acid receptor, pp2c, aba, pyl3, hormone receptor-hydrolase complex, hormone receptor/hydrolase
• Biological source: Arabidopsis thaliana (mouse-ear cress); More
• Cellular location: Cytoplasm (By similarity): Q9SSM7; Cytoplasm: Q9CAJ0
• Total number of polymer chains: 2
• Total formula weight: 61954.43

Authors

Zhang, X.,Zhang, Q.,Wang, G.,Chen, Z. (deposition date: 2012-02-18, release date: 2012-06-06, Last modification date: 2023-11-08)

Primary citation

Zhang, X.,Zhang, Q.,Xin, Q.,Yu, L.,Wang, Z.,Wu, W.,Jiang, L.,Wang, G.,Tian, W.,Deng, Z.,Wang, Y.,Liu, Z.,Long, J.,Gong, Z.,Chen, Z.
Complex Structures of the Abscisic Acid Receptor PYL3/RCAR13 Reveal a Unique Regulatory Mechanism
Structure, 20:780-790, 2012

PubMed Abstract: Abscisic acid (ABA) controls many physiological processes and mediates adaptive responses to abiotic stresses. The ABA signaling mechanisms for abscisic acid receptors PYR/PYL/RCAR (PYLs) were reported. However, it remains unclear whether the molecular mechanisms are suitable for other PYLs. Here, complex structures of PYL3 with (+)-ABA, pyrabactin and HAB1 are reported. An unexpected trans-homodimer intermediate observed in the crystal is confirmed in solution. ABA-bound PYL3 greatly promotes the generation of monomeric PYL3, which can excessively increase the efficiency of inhibiting PP2Cs. Structure-guided biochemical experiments show that Ser195 accounts for the key intermediate. Interestingly, pyrabactin binds to PYL3 in a distinct nonproductive mode with gate closure, which sheds light on the design of agonists and antagonists for abscisic acid receptors. According to different conformations of ligand-bound PYLs, the PYLs family can be divided into three subclasses, among which the trans-dimeric subclass, represented by PYL3, reveals a distinct regulatory mechanism.

PubMed: 22579247
DOI: 10.1016/j.str.2012.02.019

Experimental method

X-RAY DIFFRACTION (2.21 Å)