4DS7

Crystal structure of yeast calmodulin bound to the C-terminal fragment of spindle pole body protein Spc110

4DS7 の概要

• エントリーDOI: 10.2210/pdb4ds7/pdb
• 関連するPDBエントリー: 1LKJ
• 分子名称: Calmodulin, Spindle pole body component 110, STRONTIUM ION, ... (6 entities in total)
• 機能のキーワード: protein binding, metal binding, spindle pole body, structural protein
• 由来する生物種: Kluyveromyces lactis (Yeast); 詳細
• 細胞内の位置: Nucleus: P32380
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 91883.98

構造登録者

Klenchin, V.A.,Rayment, I. (登録日: 2012-02-18, 公開日: 2012-03-07, 最終更新日: 2024-11-20)

主引用文献

Viswanath, S.,Bonomi, M.,Kim, S.J.,Klenchin, V.A.,Taylor, K.C.,Yabut, K.C.,Umbreit, N.T.,Van Epps, H.A.,Meehl, J.,Jones, M.H.,Russel, D.,Velazquez-Muriel, J.A.,Winey, M.,Rayment, I.,Davis, T.N.,Sali, A.,Muller, E.G.
The molecular architecture of the yeast spindle pole body core determined by Bayesian integrative modeling.
Mol.Biol.Cell, 28:3298-3314, 2017

PubMed Abstract: Microtubule-organizing centers (MTOCs) form, anchor, and stabilize the polarized network of microtubules in a cell. The central MTOC is the centrosome that duplicates during the cell cycle and assembles a bipolar spindle during mitosis to capture and segregate sister chromatids. Yet, despite their importance in cell biology, the physical structure of MTOCs is poorly understood. Here we determine the molecular architecture of the core of the yeast spindle pole body (SPB) by Bayesian integrative structure modeling based on in vivo fluorescence resonance energy transfer (FRET), small-angle x-ray scattering (SAXS), x-ray crystallography, electron microscopy, and two-hybrid analysis. The model is validated by several methods that include a genetic analysis of the conserved PACT domain that recruits Spc110, a protein related to pericentrin, to the SPB. The model suggests that calmodulin can act as a protein cross-linker and Spc29 is an extended, flexible protein. The model led to the identification of a single, essential heptad in the coiled-coil of Spc110 and a minimal PACT domain. It also led to a proposed pathway for the integration of Spc110 into the SPB.

PubMed: 28814505
DOI: 10.1091/mbc.E17-06-0397

実験手法

X-RAY DIFFRACTION (2.15 Å)