4DN8

Structure of porcine surfactant protein D neck and carbohydrate recognition domain complexed with mannose

4DN8 の概要

• エントリーDOI: 10.2210/pdb4dn8/pdb
• 分子名称: Pulmonary surfactant-associated protein D, CALCIUM ION, beta-D-mannopyranose, ... (4 entities in total)
• 機能のキーワード: collectin, lectin, carbohydrate/sugar binding, lung surfactant, sugar binding protein
• 由来する生物種: Sus scrofa (pigs,swine,wild boar)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17318.31

構造登録者

van Eijk, M.,Rynkiewicz, M.J.,White, M.R.,Hartshorn, K.L.,Zou, X.,Schulten, K.,Luo, D.,Crouch, E.C.,Cafarella, T.M.,Head, J.F.,Haagsman, H.P.,Seaton, B.A. (登録日: 2012-02-08, 公開日: 2012-06-20, 最終更新日: 2024-11-20)

主引用文献

van Eijk, M.,Rynkiewicz, M.J.,White, M.R.,Hartshorn, K.L.,Zou, X.,Schulten, K.,Luo, D.,Crouch, E.C.,Cafarella, T.R.,Head, J.F.,Haagsman, H.P.,Seaton, B.A.
A Unique Sugar-binding Site Mediates the Distinct Anti-influenza Activity of Pig Surfactant Protein D.
J.Biol.Chem., 287:26666-26677, 2012

PubMed Abstract: Pigs can act as intermediate hosts by which reassorted influenza A virus (IAV) strains can be transmitted to humans and cause pandemic influenza outbreaks. The innate host defense component surfactant protein D (SP-D) interacts with glycans on the hemagglutinin of IAV and contributes to protection against IAV infection in mammals. This study shows that a recombinant trimeric neck lectin fragment derived from porcine SP-D (pSP-D) exhibits profound inhibitory activity against IAV, in contrast to comparable fragments derived from human SP-D. Crystallographic analysis of the pSP-D fragment complexed with a viral sugar component shows that a unique tripeptide loop alters the lectin site conformation of pSP-D. Molecular dynamics simulations highlight the role of this flexible loop, which adopts a more stable conformation upon sugar binding and may facilitate binding to viral glycans through contact with distal portions of the branched mannoside. The combined data demonstrate that porcine-specific structural features of SP-D contribute significantly to its distinct anti-IAV activity. These findings could help explain why pigs serve as important reservoirs for newly emerging pathogenic IAV strains.

PubMed: 22685299
DOI: 10.1074/jbc.M112.368571

実験手法

X-RAY DIFFRACTION (2.2 Å)