4DM0 の概要
| エントリーDOI | 10.2210/pdb4dm0/pdb |
| 関連するPDBエントリー | 1MUH 1MUS |
| 分子名称 | Transposase for transposon Tn5, DNA TRANSFERRED STRAND, DNA NON-TRANSFERRED STRAND, ... (6 entities in total) |
| 機能のキーワード | transposase, ribonuclease h-like motif, protein-dna complex, synaptic complex, dna recombination-dna complex, hydrolase-dna complex, hydrolase/dna |
| 由来する生物種 | Escherichia coli 詳細 |
| タンパク質・核酸の鎖数 | 3 |
| 化学式量合計 | 66173.18 |
| 構造登録者 | Klenchin, V.A.,Lovell, S.,Goryshin, I.Y.,Reznikoff, W.R.,Rayment, I. (登録日: 2012-02-06, 公開日: 2012-02-22, 最終更新日: 2023-09-13) |
| 主引用文献 | Lovell, S.,Goryshin, I.Y.,Reznikoff, W.R.,Rayment, I. Two-metal active site binding of a TN5 transposase synaptic complex Nat.Struct.Biol., 9:278-281, 2002 Cited by PubMed Abstract: A synaptic complex of Tn5 transposase with an extended outside end DNA duplex was prepared and crystallized, and its crystal structure was determined in an effort to reveal the role of metal ions in catalysis. Two Mn2+ ions bound to the active site when a single nucleotide of donor DNA was added to the 3' end of the transferred strand. Marked conformational changes were observed in the DNA bases closest to the active site. The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis. PubMed: 11896402主引用文献が同じPDBエントリー |
| 実験手法 | X-RAY DIFFRACTION (2.5 Å) |
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