4DIN

Novel Localization and Quaternary Structure of the PKA RI beta Holoenzyme

4DIN の概要

• エントリーDOI: 10.2210/pdb4din/pdb
• 関連するPDBエントリー: 2QCS
• 分子名称: cAMP-dependent protein kinase catalytic subunit alpha, cAMP-dependent protein kinase type I-beta regulatory subunit, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: isoform diversity, rib2:c2 tetrameric complex, transferase-transport protein complex, transferase/transport protein
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Cytoplasm: P05132
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84343.99

構造登録者

Ilouz, R.,Bubis, J.,Wu, J.,Yim, Y.Y.,Deal, M.S.,Kornev, A.P.,Ma, Y.,Blumenthal, D.K.,Taylor, S.S. (登録日: 2012-01-31, 公開日: 2012-07-04, 最終更新日: 2024-10-30)

主引用文献

Ilouz, R.,Bubis, J.,Wu, J.,Yim, Y.Y.,Deal, M.S.,Kornev, A.P.,Ma, Y.,Blumenthal, D.K.,Taylor, S.S.
Localization and quaternary structure of the PKA RI Beta holoenzyme
Proc.Natl.Acad.Sci.USA, 109:12443-12448, 2012

PubMed Abstract: Specificity for signaling by cAMP-dependent protein kinase (PKA) is achieved by both targeting and isoform diversity. The inactive PKA holoenzyme has two catalytic (C) subunits and a regulatory (R) subunit dimer (R(2):C(2)). Although the RIα, RIIα, and RIIβ isoforms are well studied, little is known about RIβ. We show here that RIβ is enriched selectively in mitochondria and hypothesized that its unique biological importance and functional nonredundancy will correlate with its structure. Small-angle X-ray scattering showed that the overall shape of RIβ(2):C(2) is different from its closest homolog, RIα(2):C(2). The full-length RIβ(2):C(2) crystal structure allows us to visualize all the domains of the PKA holoenzyme complex and shows how isoform-specific assembly of holoenzyme complexes can create distinct quaternary structures even though the R(1):C(1) heterodimers are similar in all isoforms. The creation of discrete isoform-specific PKA holoenzyme signaling "foci" paves the way for exploring further biological roles of PKA RIβ and establishes a paradigm for PKA signaling.

PubMed: 22797896
DOI: 10.1073/pnas.1209538109

実験手法

X-RAY DIFFRACTION (3.7 Å)