4DID

Crystal structure of Salmonella effector N-terminal domain SopB in complex with Cdc42

4DID の概要

• エントリーDOI: 10.2210/pdb4did/pdb
• 分子名称: Cell division control protein 42 homolog, Inositol phosphate phosphatase sopB, GUANOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: small gtpase, gtp binding, hydrolase-hydrolase complex, hydrolase/hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential): P60953; Secreted (By similarity): O30916
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38764.26

構造登録者

Burkinshaw, B.J.,Strynadka, N.C.J. (登録日: 2012-01-30, 公開日: 2012-02-29, 最終更新日: 2023-09-13)

主引用文献

Burkinshaw, B.J.,Prehna, G.,Worrall, L.J.,Strynadka, N.C.
Structure of Salmonella Effector Protein SopB N-terminal Domain in Complex with Host Rho GTPase Cdc42.
J.Biol.Chem., 287:13348-13355, 2012

PubMed Abstract: SopB is a type III secreted Salmonella effector protein with phosphoinositide phosphatase activity and a distinct GTPase binding domain. The latter interacts with host Cdc42, an essential Rho GTPase that regulates critical events in eukaryotic cytoskeleton organization and membrane trafficking. Structural and biochemical analysis of the SopB GTPase binding domain in complex with Cdc42 shows for the first time that SopB structurally and functionally mimics a host guanine nucleotide dissociation inhibitor (GDI) by contacting key residues in the regulatory switch regions of Cdc42 and slowing Cdc42 nucleotide exchange.

PubMed: 22362774
DOI: 10.1074/jbc.M111.331330

実験手法

X-RAY DIFFRACTION (2.3501 Å)