4DI8

CRYSTAL STRUCTURE OF THE D248A mutant of 2-PYRONE-4,6-DICARBOXYLIC ACID HYDROLASE FROM SPHINGOMONAS PAUCIMOBILIS complexed with substrate at pH 8.5

Replaces:  4D95

Summary for 4DI8

• Entry DOI: 10.2210/pdb4di8/pdb
• Related: 2QAH 4D8L 4DI9 4DIA
• Descriptor: 2-pyrone-4,6-dicarbaxylate hydrolase, 2-oxo-2H-pyran-4,6-dicarboxylic acid, ACETATE ION, ... (6 entities in total)
• Functional Keywords: hydrolase
• Biological source: Sphingomonas paucimobilis
• Total number of polymer chains: 2
• Total formula weight: 68912.66

Authors

Malashkevich, V.N.,Toro, R.,Hobbs, M.E.,Raushel, F.M.,Almo, S.C. (deposition date: 2012-01-11, release date: 2012-10-03, Last modification date: 2024-02-28)

• Primary citation: Hobbs, M.E.,Malashkevich, V.,Williams, H.J.,Xu, C.,Sauder, J.M.,Burley, S.K.,Almo, S.C.,Raushel, F.M.; Structure and Catalytic Mechanism of LigI: Insight into the Amidohydrolase Enzymes of cog3618 and Lignin Degradation.; Biochemistry, 51:3497-3507, 2012; PubMed: 22475079; DOI: 10.1021/bi300307b

Experimental method

X-RAY DIFFRACTION (1.81 Å)