4DGI

Structure of POM1 FAB fragment complexed with human PrPc Fragment 120-230

4DGI の概要

• エントリーDOI: 10.2210/pdb4dgi/pdb
• 関連するPDBエントリー: 1TQB 2W9E
• 分子名称: Major prion protein, POM1 Fab Heavy chain, POM1 Fab Light chain, ... (5 entities in total)
• 機能のキーワード: prion, antibody, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 2: Cytoplasm: P04156
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60017.31

構造登録者

Baral, P.K.,Wieland, B.,Swayampakula, M.,James, M.N. (登録日: 2012-01-26, 公開日: 2012-10-31, 最終更新日: 2024-10-30)

主引用文献

Baral, P.K.,Wieland, B.,Swayampakula, M.,Polymenidou, M.,Rahman, M.H.,Kav, N.N.,Aguzzi, A.,James, M.N.
Structural studies on the folded domain of the human prion protein bound to the Fab fragment of the antibody POM1.
Acta Crystallogr.,Sect.D, 68:1501-1512, 2012

PubMed Abstract: Prion diseases are neurodegenerative diseases characterized by the conversion of the cellular prion protein PrP(c) into a pathogenic isoform PrP(sc). Passive immunization with antiprion monoclonal antibodies can arrest the progression of prion diseases. Here, the crystal structure of the Fab fragment of an antiprion monoclonal antibody, POM1, in complex with human prion protein (huPrP(c)) has been determined to 2.4 Å resolution. The prion epitope of POM1 is in close proximity to the epitope recognized by the purportedly therapeutic antibody fragment ICSM18 Fab in complex with huPrP(c). POM1 Fab forms a 1:1 complex with huPrP(c) and the measured K(d) of 4.5 × 10(-7) M reveals moderately strong binding between them. Structural comparisons have been made among three prion-antibody complexes: POM1 Fab-huPrP(c), ICSM18 Fab-huPrP(c) and VRQ14 Fab-ovPrP(c). The prion epitopes recognized by ICSM18 Fab and VRQ14 Fab are adjacent to a prion glycosylation site, indicating possible steric hindrance and/or an altered binding mode to the glycosylated prion protein in vivo. However, both of the glycosylation sites on huPrP(c) are positioned away from the POM1 Fab binding epitope; thus, the binding mode observed in this crystal structure and the binding affinity measured for this antibody are most likely to be the same as those for the native prion protein in vivo.

PubMed: 23090399
DOI: 10.1107/S0907444912037328

実験手法

X-RAY DIFFRACTION (2.4 Å)