4D60

Structure of a dimeric Plasmodium falciparum profilin mutant

4D60 の概要

• エントリーDOI: 10.2210/pdb4d60/pdb
• 分子名称: PROFILIN, SULFATE ION (2 entities in total)
• 機能のキーワード: structural protein, actin binding, domain swapping
• 由来する生物種: Plasmodium falciparum
• 細胞内の位置: Cytoplasm, cytoskeleton : P86294
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 300859.48

構造登録者

Bhargav, S.P.,Vahokoski, J.,Kallio, J.P.,Torda, A.,Kursula, P.,Kursula, I. (登録日: 2014-11-07, 公開日: 2015-06-03, 最終更新日: 2023-12-20)

主引用文献

Bhargav, S.P.,Vahokoski, J.,Kallio, J.P.,Torda, A.E.,Kursula, P.,Kursula, I.
Two Independently Folding Units of Plasmodium Profilin Suggest Evolution Via Gene Fusion.
Cell.Mol.Life Sci., 72:4193-, 2015

PubMed Abstract: Gene fusion is a common mechanism of protein evolution that has mainly been discussed in the context of multidomain or symmetric proteins. Less is known about fusion of ancestral genes to produce small single-domain proteins. Here, we show with a domain-swapped mutant Plasmodium profilin that this small, globular, apparently single-domain protein consists of two foldons. The separation of binding sites for different protein ligands in the two halves suggests evolution via an ancient gene fusion event, analogous to the formation of multidomain proteins. Finally, the two fragments can be assembled together after expression as two separate gene products. The possibility to engineer both domain-swapped dimers and half-profilins that can be assembled back to a full profilin provides perspectives for engineering of novel protein folds, e.g., with different scaffolding functions.

PubMed: 26012696
DOI: 10.1007/S00018-015-1932-0

実験手法

X-RAY DIFFRACTION (3.3 Å)