4D2K

Crystal structure of DREP2 CIDE domain

4D2K の概要

• エントリーDOI: 10.2210/pdb4d2k/pdb
• 分子名称: DREP2 (2 entities in total)
• 機能のキーワード: apoptosis, energy metabolism, dna fragmentation factor (dff)
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 40254.16

構造登録者

Jang, T.H.,Park, H.H.,Kim, Y.G.,Jeong, J.H. (登録日: 2014-05-12, 公開日: 2015-05-27, 最終更新日: 2023-12-20)

主引用文献

Choi, J.Y.,Qiao, Q.,Hong, S.H.,Kim, C.M.,Jeong, J.H.,Kim, Y.G.,Jung, Y.K.,Wu, H.,Park, H.H.
CIDE domains form functionally important higher-order assemblies for DNA fragmentation.
Proc. Natl. Acad. Sci. U.S.A., 114:7361-7366, 2017

PubMed Abstract: Cell death-inducing DFF45-like effector (CIDE) domains, initially identified in apoptotic nucleases, form a family with diverse functions ranging from cell death to lipid homeostasis. Here we show that the CIDE domains of and human apoptotic nucleases Drep2, Drep4, and DFF40 all form head-to-tail helical filaments. Opposing positively and negatively charged interfaces mediate the helical structures, and mutations on these surfaces abolish nuclease activation for apoptotic DNA fragmentation. Conserved filamentous structures are observed in CIDE family members involved in lipid homeostasis, and mutations on the charged interfaces compromise lipid droplet fusion, suggesting that CIDE domains represent a scaffold for higher-order assembly in DNA fragmentation and other biological processes such as lipid homeostasis.

PubMed: 28652364
DOI: 10.1073/pnas.1705949114

実験手法

X-RAY DIFFRACTION (2.302 Å)