4D0L

Phosphatidylinositol 4-kinase III beta-PIK93 in a complex with Rab11a- GTP gammaS

Summary for 4D0L

• Entry DOI: 10.2210/pdb4d0l/pdb
• Related: 4D0M
• Descriptor: PHOSPHATIDYLINOSITOL 4-KINASE BETA, RAS-RELATED PROTEIN RAB-11A, N-(5-(4-CHLORO-3-(2-HYDROXY-ETHYLSULFAMOYL)- PHENYLTHIAZOLE-2-YL)-ACETAMIDE, ... (6 entities in total)
• Functional Keywords: transferase-hydrolase complex, transferase, phosphatidylinositol 4-kinase, pi4k, nucleotide, gtp, rab11, pik93, signaling, gtpase, signal transduction, golgi, recycling endosome, pi4p, phosphoinositide, ptdins4p, pi4kb, transferase/hydrolase
• Biological source: HOMO SAPIENS (HUMAN); More
• Total number of polymer chains: 6
• Total formula weight: 270715.16

Authors

Burke, J.E.,Inglis, A.J.,Perisic, O.,Masson, G.R.,McLaughin, S.H.,Rutaganira, F.,Shokat, K.M.,Williams, R.L. (deposition date: 2014-04-29, release date: 2014-05-28, Last modification date: 2023-12-20)

Primary citation

Burke, J.E.,Inglis, A.J.,Perisic, O.,Masson, G.R.,Mclaughlin, S.H.,Rutaganira, F.,Shokat, K.M.,Williams, R.L.
Structures of Pi4Kiiibeta Complexes Show Simultaneous Recruitment of Rab11 and its Effectors.
Science, 344:1035-, 2014

PubMed Abstract: Phosphatidylinositol 4-kinases (PI4Ks) and small guanosine triphosphatases (GTPases) are essential for processes that require expansion and remodeling of phosphatidylinositol 4-phosphate (PI4P)-containing membranes, including cytokinesis, intracellular development of malarial pathogens, and replication of a wide range of RNA viruses. However, the structural basis for coordination of PI4K, GTPases, and their effectors is unknown. Here, we describe structures of PI4Kβ (PI4KIIIβ) bound to the small GTPase Rab11a without and with the Rab11 effector protein FIP3. The Rab11-PI4KIIIβ interface is distinct compared with known structures of Rab complexes and does not involve switch regions used by GTPase effectors. Our data provide a mechanism for how PI4KIIIβ coordinates Rab11 and its effectors on PI4P-enriched membranes and also provide strategies for the design of specific inhibitors that could potentially target plasmodial PI4KIIIβ to combat malaria.

PubMed: 24876499
DOI: 10.1126/SCIENCE.1253397

Experimental method

X-RAY DIFFRACTION (2.94 Å)