4D0L

Phosphatidylinositol 4-kinase III beta-PIK93 in a complex with Rab11a- GTP gammaS

4D0L の概要

• エントリーDOI: 10.2210/pdb4d0l/pdb
• 関連するPDBエントリー: 4D0M
• 分子名称: PHOSPHATIDYLINOSITOL 4-KINASE BETA, RAS-RELATED PROTEIN RAB-11A, N-(5-(4-CHLORO-3-(2-HYDROXY-ETHYLSULFAMOYL)- PHENYLTHIAZOLE-2-YL)-ACETAMIDE, ... (6 entities in total)
• 機能のキーワード: transferase-hydrolase complex, transferase, phosphatidylinositol 4-kinase, pi4k, nucleotide, gtp, rab11, pik93, signaling, gtpase, signal transduction, golgi, recycling endosome, pi4p, phosphoinositide, ptdins4p, pi4kb, transferase/hydrolase
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 270715.16

構造登録者

Burke, J.E.,Inglis, A.J.,Perisic, O.,Masson, G.R.,McLaughin, S.H.,Rutaganira, F.,Shokat, K.M.,Williams, R.L. (登録日: 2014-04-29, 公開日: 2014-05-28, 最終更新日: 2025-10-01)

主引用文献

Burke, J.E.,Inglis, A.J.,Perisic, O.,Masson, G.R.,Mclaughlin, S.H.,Rutaganira, F.,Shokat, K.M.,Williams, R.L.
Structures of Pi4Kiiibeta Complexes Show Simultaneous Recruitment of Rab11 and its Effectors.
Science, 344:1035-, 2014

PubMed Abstract: Phosphatidylinositol 4-kinases (PI4Ks) and small guanosine triphosphatases (GTPases) are essential for processes that require expansion and remodeling of phosphatidylinositol 4-phosphate (PI4P)-containing membranes, including cytokinesis, intracellular development of malarial pathogens, and replication of a wide range of RNA viruses. However, the structural basis for coordination of PI4K, GTPases, and their effectors is unknown. Here, we describe structures of PI4Kβ (PI4KIIIβ) bound to the small GTPase Rab11a without and with the Rab11 effector protein FIP3. The Rab11-PI4KIIIβ interface is distinct compared with known structures of Rab complexes and does not involve switch regions used by GTPase effectors. Our data provide a mechanism for how PI4KIIIβ coordinates Rab11 and its effectors on PI4P-enriched membranes and also provide strategies for the design of specific inhibitors that could potentially target plasmodial PI4KIIIβ to combat malaria.

PubMed: 24876499
DOI: 10.1126/SCIENCE.1253397

実験手法

X-RAY DIFFRACTION (2.94 Å)