4D0G

Structure of Rab14 in complex with Rab-Coupling Protein (RCP)

4D0G の概要

• エントリーDOI: 10.2210/pdb4d0g/pdb
• 分子名称: RAS-RELATED PROTEIN RAB-14, RAB11 FAMILY-INTERACTING PROTEIN 1, GUANOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: hydrolase, rab14 gtpase, endosomal trafficking, rab- binding domain (rbd), effector recruitment
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Recycling endosome: P61106; Recycling endosome . Isoform 2: Cytoplasmic vesicle, phagosome membrane: Q6WKZ4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28335.23

構造登録者

Lall, P.,Khan, A.R. (登録日: 2014-04-25, 公開日: 2015-05-20, 最終更新日: 2024-10-16)

主引用文献

Lall, P.,Lindsay, A.J.,Hanscom, S.,Kecman, T.,Taglauer, E.S.,Mcveigh, U.M.,Franklin, E.,Mccaffrey, M.W.,Khan, A.R.
Structure-Function Analyses of the Interactions between Rab11 and Rab14 Small Gtpases with Their Shared Effector Rab Coupling Protein (Rcp).
J.Biol.Chem., 290:18817-, 2015

PubMed Abstract: Rab GTPases recruit effector proteins, via their GTP-dependent switch regions, to distinct subcellular compartments. Rab11 and Rab25 are closely related small GTPases that bind to common effectors termed the Rab11 family of interacting proteins (FIPs). The FIPs are organized into two subclasses (class I and class II) based on sequence and domain organization, and both subclasses contain a highly conserved Rab-binding domain at their C termini. Yeast two-hybrid and biochemical studies have revealed that the more distantly related Rab14 also interacts with class I FIPs. Here, we perform detailed structural, thermodynamic, and cellular analyses of the interactions between Rab14 and one of the class I FIPs, the Rab-coupling protein (RCP), to clarify the molecular aspects of the interaction. We find that Rab14 indeed binds to RCP, albeit with reduced affinity relative to conventional Rab11-FIP and Rab25-FIP complexes. However, in vivo, Rab11 recruits RCP onto biological membranes. Furthermore, biophysical analyses reveal a noncanonical 1:2 stoichiometry between Rab14-RCP in dilute solutions, in contrast to Rab11/25 complexes. The structure of Rab14-RCP reveals that Rab14 interacts with the canonical Rab-binding domain and also provides insight into the unusual properties of the complex. Finally, we show that both the Rab coupling protein and Rab14 function in neuritogenesis.

PubMed: 26032412
DOI: 10.1074/JBC.M114.612366

実験手法

X-RAY DIFFRACTION (2.5 Å)