4CZF

C. crescentus MreB, single filament, ADP

4CZF の概要

• エントリーDOI: 10.2210/pdb4czf/pdb
• 関連するPDBエントリー: 4CZE 4CZG 4CZH 4CZI 4CZJ 4CZK 4CZL 4CZM
• 分子名称: ROD SHAPE-DETERMINING PROTEIN MREB, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: structural protein, bacterial actin, bacterial cytoskeleton
• 由来する生物種: CAULOBACTER VIBRIOIDES
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37317.00

構造登録者

Lowe, J.,vandenEnt, F. (登録日: 2014-04-19, 公開日: 2014-06-11, 最終更新日: 2023-12-20)

主引用文献

Van Den Ent, F.,Izore, T.,Bharat, T.A.,Johnson, C.M.,Lowe, J.
Bacterial Actin Mreb Forms Antiparallel Double Filaments.
Elife, 3:02634-, 2014

PubMed Abstract: Filaments of all actin-like proteins known to date are assembled from pairs of protofilaments that are arranged in a parallel fashion, generating polarity. In this study, we show that the prokaryotic actin homologue MreB forms pairs of protofilaments that adopt an antiparallel arrangement in vitro and in vivo. We provide an atomic view of antiparallel protofilaments of Caulobacter MreB as apparent from crystal structures. We show that a protofilament doublet is essential for MreB's function in cell shape maintenance and demonstrate by in vivo site-specific cross-linking the antiparallel orientation of MreB protofilaments in E. coli. 3D cryo-EM shows that pairs of protofilaments of Caulobacter MreB tightly bind to membranes. Crystal structures of different nucleotide and polymerisation states of Caulobacter MreB reveal conserved conformational changes accompanying antiparallel filament formation. Finally, the antimicrobial agents A22/MP265 are shown to bind close to the bound nucleotide of MreB, presumably preventing nucleotide hydrolysis and destabilising double protofilaments.DOI: http://dx.doi.org/10.7554/eLife.02634.001.

PubMed: 24843005
DOI: 10.7554/ELIFE.02634

実験手法

X-RAY DIFFRACTION (1.64 Å)