4CWC

Structure of Rolling Circle Replication Initiator Protein (RepDE) from Staphylococcus aureus

4CWC の概要

• エントリーDOI: 10.2210/pdb4cwc/pdb
• 関連するPDBエントリー: 4CWE
• 分子名称: REPLICATION INITIATION PROTEIN, REPLICATION INITIATION PROTEIN (1 entity in total)
• 機能のキーワード: isomerase, antibiotic resistance, relaxase, pcra helicase, chimera
• 由来する生物種: STAPHYLOCOCCUS AUREUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68560.23

構造登録者

Carr, S.B.,Phillips, S.E.V.,Thomas, C.D. (登録日: 2014-04-02, 公開日: 2015-04-15, 最終更新日: 2023-12-20)

主引用文献

Carr, S.B.,Phillips, S.E.,Thomas, C.D.
Structures of Replication Initiation Proteins from Staphylococcal Antibiotic Resistance Plasmids Reveal Protein Asymmetry and Flexibility are Necessary for Replication.
Nucleic Acids Res., 44:2417-, 2016

PubMed Abstract: Antibiotic resistance in pathogenic bacteria is a continual threat to human health, often residing in extrachromosomal plasmid DNA. Plasmids of the pT181 family are widespread and confer various antibiotic resistances to Staphylococcus aureus. They replicate via a rolling circle mechanism that requires a multi-functional, plasmid-encoded replication protein to initiate replication, recruit a helicase to the site of initiation and terminate replication after DNA synthesis is complete. We present the first atomic resolution structures of three such replication proteins that reveal distinct, functionally relevant conformations. The proteins possess a unique active site and have been shown to contain a catalytically essential metal ion that is bound in a manner distinct from that of any other rolling circle replication proteins. These structures are the first examples of the Rep_trans Pfam family providing insights into the replication of numerous antibiotic resistance plasmids from Gram-positive bacteria, Gram-negative phage and the mobilisation of DNA by conjugative transposons.

PubMed: 26792891
DOI: 10.1093/NAR/GKV1539

実験手法

X-RAY DIFFRACTION (2.9 Å)