4CVD

Crystal structure of the central repeat of cell wall binding module of Cpl7

Summary for 4CVD

• Entry DOI: 10.2210/pdb4cvd/pdb
• Descriptor: LYSOZYME (2 entities in total)
• Functional Keywords: hydrolase, streptococcus pneumoniae
• Biological source: STREPTOCOCCUS PHAGE CP-7
• Total number of polymer chains: 1
• Total formula weight: 5263.68

Authors

Silva-Martin, N.,Uson, I.,Rodriguez, D.D.,Hermoso, J.A. (deposition date: 2014-03-25, release date: 2015-04-08, Last modification date: 2024-05-08)

Primary citation

Bustamante, N.,Iglesias-Bexiga, M.,Bernardo-Garcia, N.,Silva-Martin, N.,Garcia, G.,Campanero-Rhodes, M.A.,Garcia, E.,Uson, I.,Buey, R.M.,Garcia, P.,Hermoso, J.A.,Bruix, M.,Menendez, M.
Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan.
Sci Rep, 7:16494-16494, 2017

PubMed Abstract: Endolysins, the cell wall lytic enzymes encoded by bacteriophages to release the phage progeny, are among the top alternatives to fight against multiresistant pathogenic bacteria; one of the current biggest challenges to global health. Their narrow range of susceptible bacteria relies, primarily, on targeting specific cell-wall receptors through specialized modules. The cell wall-binding domain of Cpl-7 endolysin, made of three CW_7 repeats, accounts for its extended-range of substrates. Using as model system the cell wall-binding domain of Cpl-7, here we describe the molecular basis for the bacterial cell wall recognition by the CW_7 motif, which is widely represented in sequences of cell wall hydrolases. We report the crystal and solution structure of the full-length domain, identify N-acetyl-D-glucosaminyl-(β1,4)-N-acetylmuramyl-L-alanyl-D-isoglutamine (GMDP) as the peptidoglycan (PG) target recognized by the CW_7 motifs, and characterize feasible GMDP-CW_7 contacts. Our data suggest that Cpl-7 cell wall-binding domain might simultaneously bind to three PG chains, and also highlight the potential use of CW_7-containing lysins as novel anti-infectives.

PubMed: 29184076
DOI: 10.1038/s41598-017-16392-4

Experimental method

X-RAY DIFFRACTION (1.666 Å)