4CQK

Crystal structure of ligand-bound NaD1

4CQK の概要

• エントリーDOI: 10.2210/pdb4cqk/pdb
• 分子名称: FLOWER-SPECIFIC DEFENSIN, [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: plant protein, innate immunity
• 由来する生物種: NICOTIANA ALATA (PERSIAN TOBACCO)
• 細胞内の位置: Secreted (By similarity): Q8GTM0
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 87261.16

構造登録者

Lay, F.T.,Mills, G.M.,Poon, I.K.H.,Baxter, A.A.,Hulett, M.D.,Kvansakul, M. (登録日: 2014-02-17, 公開日: 2014-04-16, 最終更新日: 2024-11-13)

主引用文献

Poon, I.K.H.,Baxter, A.A.,Lay, F.T.,Mills, G.D.,Adda, C.G.,Payne, J.A.,Phan, T.K.,Ryan, G.F.,White, J.A.,Veneer, P.K.,Van Der Weerden, N.L.,Anderson, M.A.,Kvansakul, M.,Hulett, M.D.
Phosphoinositide-Mediated Oligomerization of a Defensin Induces Cell Lysis.
Elife, 3:1808-, 2014

PubMed Abstract: Cationic antimicrobial peptides (CAPs) such as defensins are ubiquitously found innate immune molecules that often exhibit broad activity against microbial pathogens and mammalian tumor cells. Many CAPs act at the plasma membrane of cells leading to membrane destabilization and permeabilization. In this study, we describe a novel cell lysis mechanism for fungal and tumor cells by the plant defensin NaD1 that acts via direct binding to the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2). We determined the crystal structure of a NaD1:PIP2 complex, revealing a striking oligomeric arrangement comprising seven dimers of NaD1 that cooperatively bind the anionic headgroups of 14 PIP2 molecules through a unique 'cationic grip' configuration. Site-directed mutagenesis of NaD1 confirms that PIP2-mediated oligomerization is important for fungal and tumor cell permeabilization. These observations identify an innate recognition system by NaD1 for direct binding of PIP2 that permeabilizes cells via a novel membrane disrupting mechanism. DOI: http://dx.doi.org/10.7554/eLife.01808.001.

PubMed: 24692446
DOI: 10.7554/ELIFE.01808

実験手法

X-RAY DIFFRACTION (1.6 Å)