4COV

Crystal Structure of Epithelial Adhesin 6 A domain (Epa6A) from Candida glabrata in complex with Gala1-3Gal

4COV の概要

• エントリーDOI: 10.2210/pdb4cov/pdb
• 関連するPDBエントリー: 4COU 4COW 4COY 4COZ 4CP0 4CP1 4CP2
• 分子名称: EPITHELIAL ADHESIN 6, alpha-D-galactopyranose-(1-3)-beta-D-galactopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: cell adhesion, lectin, tissue invasion, pathogenicity
• 由来する生物種: CANDIDA GLABRATA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30379.36

構造登録者

Kock, M.,Maestre-Reyna, M.,Diderrich, R.,Moesch, H.-U.,Essen, L.-O. (登録日: 2014-01-31, 公開日: 2015-02-18, 最終更新日: 2024-11-20)

主引用文献

Diderrich, R.,Kock, M.,Maestre-Reyna, M.,Rupp, S.,Essen, L.-O.,Moesch, H.-U.
Structural Hotspots Determine Functional Diversity of the Candida Glabrata Epithelial Adhesin Family
J.Biol.Chem., 290:19597-, 2015

PubMed Abstract: For host colonization, the human fungal pathogen Candida glabrata is known to utilize a large family of highly related surface-exposed cell wall proteins, the lectin-like epithelial adhesins (Epas). To reveal the structure-function relationships within the entire Epa family, we have performed a large scale functional analysis of the adhesion (A) domains of 17 Epa paralogs in combination with three-dimensional structural studies of selected members with cognate ligands. Our study shows that most EpaA domains exert lectin-like functions and together recognize a wide variety of glycans with terminal galactosides for conferring epithelial cell adhesion. We further identify several conserved and variable structural features within the diverse Epa ligand binding pockets, which affect affinity and specificity. These features rationalize why mere phylogenetic relationships within the Epa family are weak indicators for functional classification and explain how Epa-like adhesins have evolved in C. glabrata and related fungal species.

PubMed: 26105055
DOI: 10.1074/JBC.M115.655654

実験手法

X-RAY DIFFRACTION (1.5 Å)