4CKP

Structure of an N-terminal fragment of Leishmania SAS-6 that contains part of its coiled coil domain

Summary for 4CKP

• Entry DOI: 10.2210/pdb4ckp/pdb
• Related: 4CKM 4CKN
• Descriptor: SAS-6 (1 entity in total)
• Functional Keywords: structural protein, basal body, centriole, cartwheel, trypanosomatids
• Biological source: LEISHMANIA MAJOR
• Total number of polymer chains: 6
• Total formula weight: 149617.21

Authors

van Breugel, M. (deposition date: 2014-01-07, release date: 2014-03-05, Last modification date: 2023-12-20)

Primary citation

van Breugel, M.,Wilcken, R.,McLaughlin, S.H.,Rutherford, T.J.,Johnson, C.M.
Structure of the SAS-6 cartwheel hub from Leishmania major.
Elife, 3:e01812-e01812, 2014

PubMed Abstract: Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole symmetry is unclear. Here we show that Leishmania major SAS-6 crystallizes as a 9-fold symmetric cartwheel and provide the X-ray structure of this assembly at a resolution of 3.5 Å. We furthermore demonstrate that oligomerization of Leishmania SAS-6 can be inhibited by a small molecule in vitro and provide indications for its binding site. Our results firmly establish that SAS-6 can impose cartwheel symmetry on its own and indicate how this process might occur mechanistically in vivo. Importantly, our data also provide a proof-of-principle that inhibition of SAS-6 oligomerization by small molecules is feasible. DOI: http://dx.doi.org/10.7554/eLife.01812.001.

PubMed: 24596152
DOI: 10.7554/eLife.01812

Experimental method

X-RAY DIFFRACTION (3.45 Å)