4CKM

Structure of the N-terminal domain of Leishmania SAS-6

4CKM の概要

• エントリーDOI: 10.2210/pdb4ckm/pdb
• 関連するPDBエントリー: 4CKN 4CKP
• 分子名称: SAS-6, MAGNESIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: structural protein, basal body, centriole, cartwheel, trypanosomatids
• 由来する生物種: LEISHMANIA MAJOR
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 59181.68

構造登録者

van Breugel, M. (登録日: 2014-01-07, 公開日: 2014-03-05, 最終更新日: 2024-05-08)

主引用文献

van Breugel, M.,Wilcken, R.,McLaughlin, S.H.,Rutherford, T.J.,Johnson, C.M.
Structure of the SAS-6 cartwheel hub from Leishmania major.
Elife, 3:e01812-e01812, 2014

PubMed Abstract: Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole symmetry is unclear. Here we show that Leishmania major SAS-6 crystallizes as a 9-fold symmetric cartwheel and provide the X-ray structure of this assembly at a resolution of 3.5 Å. We furthermore demonstrate that oligomerization of Leishmania SAS-6 can be inhibited by a small molecule in vitro and provide indications for its binding site. Our results firmly establish that SAS-6 can impose cartwheel symmetry on its own and indicate how this process might occur mechanistically in vivo. Importantly, our data also provide a proof-of-principle that inhibition of SAS-6 oligomerization by small molecules is feasible. DOI: http://dx.doi.org/10.7554/eLife.01812.001.

PubMed: 24596152
DOI: 10.7554/eLife.01812

実験手法

X-RAY DIFFRACTION (2.15 Å)