4CFP

Crystal structure of MltC in complex with tetrasaccharide at 2.15 A resolution

4CFP の概要

• エントリーDOI: 10.2210/pdb4cfp/pdb
• 関連するPDBエントリー: 4CFO
• 分子名称: MEMBRANE-BOUND LYTIC MUREIN TRANSGLYCOSYLASE C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-N-acetyl-beta-muramic acid-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-beta-D-glucopyranoside, HEXAETHYLENE GLYCOL, ... (5 entities in total)
• 機能のキーワード: lyase
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell outer membrane; Lipid-anchor (By similarity): C5A0N2
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78970.47

構造登録者

Bernardo-Garcia, N.,Artola-Recolons, C.,Hermoso, J.A. (登録日: 2013-11-19, 公開日: 2014-07-23, 最終更新日: 2024-05-08)

主引用文献

Artola-Recolons, C.,Lee, M.,Bernardo-Garcia, N.,Blazquez, B.,Hesek, D.,Bartual, S.G.,Mahasenan, K.V.,Lastochkin, E.,Pi, H.,Boggess, B.,Meindl, K.,Uson, I.,Fisher, J.F.,Mobashery, S.,Hermoso, J.A.
Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase Mltc of Escherichia Coli.
Acs Chem.Biol., 9:2058-, 2014

PubMed Abstract: The lytic transglycosylases are essential bacterial enzymes that catalyze the nonhydrolytic cleavage of the glycan strands of the bacterial cell wall. We describe here the structural and catalytic properties of MltC, one of the seven lytic transglycosylases found in the genome of the Gram-negative bacterium Escherichia coli. The 2.3 Å resolution X-ray structure of a soluble construct of MltC shows a unique, compared to known lytic transglycosylase structures, two-domain structure characterized by an expansive active site of 53 Å length extending through an interface between the domains. The structures of three complexes of MltC with cell wall analogues suggest the positioning of the peptidoglycan in the active site both as a substrate and as a product. One complex is suggested to correspond to an intermediate in the course of sequential and exolytic cleavage of the peptidoglycan. Moreover, MltC partitioned its reactive oxocarbenium-like intermediate between trapping by the C6-hydroxyl of the muramyl moiety (lytic transglycosylase activity, the major path) and by water (muramidase activity). Genomic analysis identifies the presence of an MltC homologue in no less than 791 bacterial genomes. While the role of MltC in cell wall assembly and maturation remains uncertain, we propose a functional role for this enzyme as befits the uniqueness of its two-domain structure.

PubMed: 24988330
DOI: 10.1021/CB500439C

実験手法

X-RAY DIFFRACTION (2.15 Å)