4CB8

Structural and mutational analysis reveals that CTNNBL1 binds NLSs in a manner distinct from that of its closest armadillo-relative, karyopherin alpha

4CB8 の概要

• エントリーDOI: 10.2210/pdb4cb8/pdb
• 関連するPDBエントリー: 4CB9 4CBA
• 分子名称: BETA-CATENIN-LIKE PROTEIN 1, SULFATE ION (3 entities in total)
• 機能のキーワード: apoptosis, import, aid, deaminase
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus: Q8WYA6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57407.83

構造登録者

Ganesh, K.,vanMaldegem, F.,Telerman, S.B.,Simpson, P.,Johnson, C.M.,Williams, R.L.,Neuberger, M.S.,Rada, C. (登録日: 2013-10-10, 公開日: 2013-12-04, 最終更新日: 2024-05-08)

主引用文献

Ganesh, K.,Maldegem, F.V.,Telerman, S.B.,Simpson, P.,Johnson, C.M.,Williams, R.L.,Neuberger, M.S.,Rada, C.
Structural and Mutational Analysis Reveals that Ctnnbl1 Binds Nlss in a Manner Distinct from that of its Closest Armadillo-Relative, Karyopherin Alpha
FEBS Lett., 588:21-, 2014

PubMed Abstract: CTNNBL1 is a spliceosome-associated protein that binds nuclear localization signals (NLSs) in splice factors CDC5L and Prp31 as well as the antibody diversifying enzyme AID. Here, crystal structures of human CTNNBL1 reveal a distinct structure from its closest homologue karyopherin-α. CTNNBL1 comprises a HEAT-like domain (including a nuclear export signal), a central armadillo domain, and a coiled-coil C-terminal domain. Structure-guided mutations of the region homologous to the karyopherin-α NLS-binding site fail to disrupt CTNNBL1-NLS interactions. Our results identify CTNNBL1 as a unique selective NLS-binding protein with striking differences from karyopherin-αs.

PubMed: 24269683
DOI: 10.1016/J.FEBSLET.2013.11.013

実験手法

X-RAY DIFFRACTION (2.9 Å)