4CAS

Serial femtosecond crystallography structure of a photosynthetic reaction center

4CAS の概要

• エントリーDOI: 10.2210/pdb4cas/pdb
• 分子名称: PHOTOSYNTHETIC REACTION CENTER CYTOCHROME C SUBUNIT, FE (II) ION, MENAQUINONE-7, ... (14 entities in total)
• 機能のキーワード: photosynthesis, lipidic-sponge phase, reaction center, electron transport, cell membrane, metal- binding, transmembrane, chromophore
• 由来する生物種: BLASTOCHLORIS VIRIDIS; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 146387.98

構造登録者

Johansson, L.C.,Arnlund, D.,Katona, G.,White, T.A.,Barty, A.,DePonte, D.P.,Shoeman, R.L.,Wickstrand, C.,Sharma, A.,Williams, G.J.,Aquila, A.,Bogan, M.J.,Caleman, C.,Davidsson, J.,Doak, R.B.,Frank, M.,Fromme, R.,Galli, L.,Grotjohann, I.,Hunter, M.S.,Kassemeyer, S.,Kirian, R.A.,Kupitz, C.,Liang, M.,Lomb, L.,Malmerberg, E.,Martin, A.V.,Messerschmidt, M.,Nass, K.,Redecke, L.,Seibert, M.M.,Sjohamn, J.,Steinbrener, J.,Stellato, F.,Wang, D.,Wahlgren, W.Y.,Weierstall, U.,Westenhoff, S.,Zatsepin, N.A.,Boutet, S.,Spence, J.C.H.,Schlichting, I.,Chapman, H.N.,Fromme, P.,Neutze, R. (登録日: 2013-10-09, 公開日: 2013-12-25, 最終更新日: 2023-12-20)

主引用文献

Johansson, L.C.,Arnlund, D.,Katona, G.,White, T.A.,Barty, A.,DePonte, D.P.,Shoeman, R.L.,Wickstrand, C.,Sharma, A.,Williams, G.J.,Aquila, A.,Bogan, M.J.,Caleman, C.,Davidsson, J.,Doak, R.B.,Frank, M.,Fromme, R.,Galli, L.,Grotjohann, I.,Hunter, M.S.,Kassemeyer, S.,Kirian, R.A.,Kupitz, C.,Liang, M.,Lomb, L.,Malmerberg, E.,Martin, A.V.,Messerschmidt, M.,Nass, K.,Redecke, L.,Seibert, M.M.,Sjohamn, J.,Steinbrener, J.,Stellato, F.,Wang, D.,Wahlgren, W.Y.,Weierstall, U.,Westenhoff, S.,Zatsepin, N.A.,Boutet, S.,Spence, J.C.,Schlichting, I.,Chapman, H.N.,Fromme, P.,Neutze, R.
Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography.
Nat Commun, 4:2911-2911, 2013

PubMed Abstract: Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

PubMed: 24352554
DOI: 10.1038/ncomms3911

実験手法

X-RAY DIFFRACTION (3.5 Å)