4CA0

Structural Basis for the microtubule binding of the human kinetochore Ska complex

Summary for 4CA0

• Entry DOI: 10.2210/pdb4ca0/pdb
• Related: 4C9Y
• Descriptor: SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1 (2 entities in total)
• Functional Keywords: cell cycle, cell divison, kinetochore-microtubule attachment, winged-helix domain
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Cytoplasm, cytoskeleton, spindle: Q96BD8
• Total number of polymer chains: 2
• Total formula weight: 29288.22

Authors

Abad, M.,Medina, B.,Santamaria, A.,Zou, J.,Plasberg-Hill, C.,Madhumalar, A.,Jayachandran, U.,Redli, P.M.,Rappsilber, J.,Nigg, E.A.,Jeyaprakash, A.A. (deposition date: 2013-10-04, release date: 2014-01-22, Last modification date: 2024-11-06)

Primary citation

Abad, M.A.,Medina, B.,Santamaria, A.,Zou, J.,Plasberg-Hill, C.,Madhumalar, A.,Jayachandran, U.,Redli, P.M.,Rappsilber, J.,Nigg, E.A.,Jeyaprakash, A.A.
Structural Basis for Microtubule Recognition by the Human Kinetochore Ska Complex.
Nat.Commun., 5:2964-, 2014

PubMed Abstract: The ability of kinetochores (KTs) to maintain stable attachments to dynamic microtubule structures ('straight' during microtubule polymerization and 'curved' during microtubule depolymerization) is an essential requirement for accurate chromosome segregation. Here we show that the kinetochore-associated Ska complex interacts with tubulin monomers via the carboxy-terminal winged-helix domain of Ska1, providing the structural basis for the ability to bind both straight and curved microtubule structures. This contrasts with the Ndc80 complex, which binds straight microtubules by recognizing the dimeric interface of tubulin. The Ska1 microtubule-binding domain interacts with tubulins using multiple contact sites that allow the Ska complex to bind microtubules in multiple modes. Disrupting either the flexibility or the tubulin contact sites of the Ska1 microtubule-binding domain perturbs normal mitotic progression, explaining the critical role of the Ska complex in maintaining a firm grip on dynamic microtubules.

PubMed: 24413531
DOI: 10.1038/NCOMMS3964

Experimental method

X-RAY DIFFRACTION (2.259 Å)