4CA0

Structural Basis for the microtubule binding of the human kinetochore Ska complex

4CA0 の概要

• エントリーDOI: 10.2210/pdb4ca0/pdb
• 関連するPDBエントリー: 4C9Y
• 分子名称: SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 1 (2 entities in total)
• 機能のキーワード: cell cycle, cell divison, kinetochore-microtubule attachment, winged-helix domain
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm, cytoskeleton, spindle: Q96BD8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29288.22

構造登録者

Abad, M.,Medina, B.,Santamaria, A.,Zou, J.,Plasberg-Hill, C.,Madhumalar, A.,Jayachandran, U.,Redli, P.M.,Rappsilber, J.,Nigg, E.A.,Jeyaprakash, A.A. (登録日: 2013-10-04, 公開日: 2014-01-22, 最終更新日: 2024-11-06)

主引用文献

Abad, M.A.,Medina, B.,Santamaria, A.,Zou, J.,Plasberg-Hill, C.,Madhumalar, A.,Jayachandran, U.,Redli, P.M.,Rappsilber, J.,Nigg, E.A.,Jeyaprakash, A.A.
Structural Basis for Microtubule Recognition by the Human Kinetochore Ska Complex.
Nat.Commun., 5:2964-, 2014

PubMed Abstract: The ability of kinetochores (KTs) to maintain stable attachments to dynamic microtubule structures ('straight' during microtubule polymerization and 'curved' during microtubule depolymerization) is an essential requirement for accurate chromosome segregation. Here we show that the kinetochore-associated Ska complex interacts with tubulin monomers via the carboxy-terminal winged-helix domain of Ska1, providing the structural basis for the ability to bind both straight and curved microtubule structures. This contrasts with the Ndc80 complex, which binds straight microtubules by recognizing the dimeric interface of tubulin. The Ska1 microtubule-binding domain interacts with tubulins using multiple contact sites that allow the Ska complex to bind microtubules in multiple modes. Disrupting either the flexibility or the tubulin contact sites of the Ska1 microtubule-binding domain perturbs normal mitotic progression, explaining the critical role of the Ska complex in maintaining a firm grip on dynamic microtubules.

PubMed: 24413531
DOI: 10.1038/NCOMMS3964

実験手法

X-RAY DIFFRACTION (2.259 Å)