4C9C

Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 1E protein (Form A)

4C9C の概要

• エントリーDOI: 10.2210/pdb4c9c/pdb
• 関連するPDBエントリー: 4C94
• 分子名称: MAJOR STRAWBERRY ALLERGEN FRA A 1-E, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: start, pyr/pyl/rcar, allergen, bet v 1
• 由来する生物種: FRAGARIA X ANANASSA (STRAWBERRY)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36250.91

構造登録者

Casanal, A.,Zander, U.,Valpuesta, V.,Marquez, J.A. (登録日: 2013-10-02, 公開日: 2013-10-16, 最終更新日: 2023-12-20)

主引用文献

Casanal, A.,Zander, U.,Munoz, C.,Dupeux, F.,Luque, I.,Botella, M.A.,Schwab, W.,Valpuesta, V.,Marquez, J.A.
The Strawberry Pathogenesis-Related 10 (Pr-10) Fra a Proteins Control Flavonoid Biosynthesis by Binding to Metabolic Intermediates.
J.Biol.Chem., 288:35322-, 2013

PubMed Abstract: Pathogenesis-related 10 (PR-10) proteins are involved in many aspects of plant biology but their molecular function is still unclear. They are related by sequence and structural homology to mammalian lipid transport and plant abscisic acid receptor proteins and are predicted to have cavities for ligand binding. Recently, three new members of the PR-10 family, the Fra a proteins, have been identified in strawberry, where they are required for the activity of the flavonoid biosynthesis pathway, which is essential for the development of color and flavor in fruits. Here, we show that Fra a proteins bind natural flavonoids with different selectivity and affinities in the low μm range. The structural analysis of Fra a 1 E and a Fra a 3-catechin complex indicates that loops L3, L5, and L7 surrounding the ligand-binding cavity show significant flexibility in the apo forms but close over the ligand in the Fra a 3-catechin complex. Our findings provide mechanistic insight on the function of Fra a proteins and suggest that PR-10 proteins, which are widespread in plants, may play a role in the control of secondary metabolic pathways by binding to metabolic intermediates.

PubMed: 24133217
DOI: 10.1074/JBC.M113.501528

実験手法

X-RAY DIFFRACTION (2.2 Å)