4C83

Crystal Structure of the IgG2a LPT3 in complex with an 8-sugar inner core analogue of Neisseria meningitidis

4C83 の概要

• エントリーDOI: 10.2210/pdb4c83/pdb
• 分子名称: LPT3 HEAVY CHAIN, LPT3 LIGHT CHAIN, 2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-2)-L-glycero-alpha-D-manno-heptopyranose-(1-3)-[beta-D-glucopyranose-(1-4)]L-glycero-alpha-D-manno-heptopyranose-(1-5)-3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid, ... (5 entities in total)
• 機能のキーワード: immune system, lipooligosaccharides, antibodies, antigen-antibody complex
• 由来する生物種: MUS MUSCULUS (HOUSE MOUSE); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 96516.96

構造登録者

Parker, M.J.,Gomery, K.,Richard, G.,Mackenzie, C.R.,Cox, A.D.,Richards, J.C.,Evans, S.V. (登録日: 2013-09-29, 公開日: 2014-02-12, 最終更新日: 2024-11-06)

主引用文献

Parker, M.J.,Gomery, K.,Richard, G.,Mackenzie, C.R.,Cox, A.D.,Richards, J.C.,Evans, S.V.
Structural Basis for Selective Cross-Reactivity in a Bactericidal Antibody Against Inner Core Lipooligosaccharide from Neisseria Meningitidis.
Glycobiology, 24:442-, 2014

PubMed Abstract: The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an inner core N-acetylglucosamine residue unique to N. meningitidis and does not include the lipid A moiety, which is disordered in the structure, but is positioned to allow the binding of free and membrane-anchored full-length LOS. All the amino acid residues that contact antigen are of germline origin but, remarkably, two consecutive somatic mutations of serine to glycine in the heavy chain at residues 52 and 52a are positioned to deprive the antibody of advantageous interactions and so weaken binding. However, these mutations are key to allowing selective cross-reactivity with the HepII-3-PEtn inner core variant expressed by 70% of strains. Neisseria meningitidis is a leading cause of disease in the developed world and is especially dangerous to children, who lack the necessary protective antibodies. The structure of Fab LPT3-1 in complex with LOS provides insight into the antibody's selective ability to recognize multiple clinically relevant variations of the LOS inner core from N. meningitidis.

PubMed: 24488440
DOI: 10.1093/GLYCOB/CWU009

実験手法

X-RAY DIFFRACTION (2.69 Å)