4C7W

Crystal structure of Mouse Hepatitis virus strain S Hemagglutinin- esterase in complex with 4-O-acetylated sialic acid

4C7W の概要

• エントリーDOI: 10.2210/pdb4c7w/pdb
• 関連するPDBエントリー: 4C7L
• 分子名称: HEMAGGLUTININ-ESTERASE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: hydrolase, receptor destroying, receptor binding
• 由来する生物種: MURINE HEPATITIS VIRUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93568.91

構造登録者

Zeng, Q.H.,Huizinga, E.G. (登録日: 2013-09-26, 公開日: 2013-10-16, 最終更新日: 2024-10-23)

主引用文献

Langereis, M.A.,Zeng, Q.,Heesters, B.A.,Huizinga, E.G.,De Groot, R.J.
The Murine Coronavirus Hemagglutinin-Esterase Receptor-Binding Site: A Major Shift in Ligand Specificity Through Modest Changes in Architecture.
Plos Pathog., 8:02492-, 2012

PubMed Abstract: The hemagglutinin-esterases (HEs), envelope glycoproteins of corona-, toro- and orthomyxoviruses, mediate reversible virion attachment to O-acetylated sialic acids (O-Ac-Sias). They do so through concerted action of distinct receptor-binding ("lectin") and receptor-destroying sialate O-acetylesterase ("esterase") domains. Most HEs target 9-O-acetylated Sias. In one lineage of murine coronaviruses, however, HE esterase substrate and lectin ligand specificity changed dramatically as these viruses evolved to use 4-O-acetylated Sias instead. Here we present the crystal structure of the lectin domain of mouse hepatitis virus (MHV) strain S HE, resolved both in its native state and in complex with a receptor analogue. The data show that the shift from 9-O- to 4-O-Ac-Sia receptor usage primarily entailed a change in ligand binding topology and, surprisingly, only modest changes in receptor-binding site architecture. Our findings illustrate the ease with which viruses can change receptor-binding specificity with potential consequences for host-, organ and/or cell tropism, and for pathogenesis.

PubMed: 22291594
DOI: 10.1371/JOURNAL.PPAT.1002492

実験手法

X-RAY DIFFRACTION (2.5 Å)