4C5Q

measles virus phosphoprotein tetramerization domain

4C5Q の概要

• エントリーDOI: 10.2210/pdb4c5q/pdb
• 分子名称: PHOSPHOPROTEIN (2 entities in total)
• 機能のキーワード: viral protein, oligomerization domain
• 由来する生物種: MEASLES VIRUS
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 72458.48

構造登録者

Blocquel, D.,Habchi, J.,Durand, E.,Sevajol, M.,Ferron, F.,Papageorgiou, N.,Longhi, S. (登録日: 2013-09-14, 公開日: 2014-04-09, 最終更新日: 2023-12-20)

主引用文献

Blocquel, D.,Habchi, J.,Durand, E.,Sevajol, M.,Ferron, F.,Erales, J.,Papageorgiou, N.,Longhi, S.
Coiled-Coil Deformations in Crystal Structures: The Measles Virus Phosphoprotein Multimerization Domain as an Illustrative Example.
Acta Crystallogr.,Sect.D, 70:1589-, 2014

PubMed Abstract: The structures of two constructs of the measles virus (MeV) phosphoprotein (P) multimerization domain (PMD) are reported and are compared with a third structure published recently by another group [Communie et al. (2013), J. Virol. 87, 7166-7169]. Although the three structures all have a tetrameric and parallel coiled-coil arrangement, structural comparison unveiled considerable differences in the quaternary structure and unveiled that the three structures suffer from significant structural deformation induced by intermolecular interactions within the crystal. These results show that crystal packing can bias conclusions about function and mechanism based on analysis of a single crystal structure, and they challenge to some extent the assumption according to which coiled-coil structures can be reliably predicted from the amino-acid sequence. Structural comparison also highlighted significant differences in the extent of disorder in the C-terminal region of each monomer. The differential flexibility of the C-terminal region is also supported by size-exclusion chromatography and small-angle X-ray scattering studies, which showed that MeV PMD exists in solution as a dynamic equilibrium between two tetramers of different compaction. Finally, the possible functional implications of the flexibility of the C-terminal region of PMD are discussed.

PubMed: 24914970
DOI: 10.1107/S139900471400234X

実験手法

X-RAY DIFFRACTION (2.2 Å)