4C4W

Structure of a rare, non-standard sequence k-turn bound by L7Ae protein

4C4W の概要

• エントリーDOI: 10.2210/pdb4c4w/pdb
• 関連するPDBエントリー: 4C40
• 分子名称: U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A, 50S RIBOSOMAL PROTEIN L7AE, TSKT-23, ... (4 entities in total)
• 機能のキーワード: rna binding protein-rna complex, rna binding protein, kink turn, rna binding protein/rna
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: P09012
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 96595.42

構造登録者

Huang, L.,Lilley, D.M.J. (登録日: 2013-09-09, 公開日: 2014-02-12, 最終更新日: 2023-12-20)

主引用文献

Huang, L.,Lilley, D.M.J.
Structure of a Rare, Non-Standard Sequence K-Turn Bound by L7Ae Protein
Nucleic Acids Res., 42:4734-, 2014

PubMed Abstract: Kt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D and H/ACA small nucleolar RNAs and U4 small nuclear RNA. We have solved the crystal structure of T. solenopsae Kt-23 RNA bound to Archeoglobus fulgidus L7Ae protein at a resolution of 2.95 Å. The protein binds in the major groove displayed on the outer face of the k-turn, in a manner similar to complexes with standard k-turn structures. The k-turn adopts a standard N3 class conformation, with a single hydrogen bond from A2b N6 to A2n N3. This contrasts with the structure of the same sequence located in the SAM-I riboswitch, where it adopts an N1 structure, showing the inherent plasticity of k-turn structure. This potentially can affect any tertiary interactions in which the RNA participates.

PubMed: 24482444
DOI: 10.1093/NAR/GKU087

実験手法

X-RAY DIFFRACTION (2.95 Å)