4C4D

Covalent glycosyl-enzyme intermediate of Hypocrea jecorina Cel7a E217Q mutant trapped using DNP-2-deoxy-2-fluoro-cellotrioside

4C4D の概要

• エントリーDOI: 10.2210/pdb4c4d/pdb
• 関連するPDBエントリー: 4C4C
• 関連するBIRD辞書のPRD_ID: PRD_900005
• 分子名称: CELLULOSE 1,4-BETA-CELLOBIOSIDASE, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-2-deoxy-2-fluoro-alpha-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: hydrolase, glycoside hydrolase, cellobiohydrolase, cellulase.
• 由来する生物種: TRICHODERMA REESEI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48128.24

構造登録者

Haddad-Momeni, M.,Mackenzie, L.,Sandgren, M.,Withers, S.G.,Stahlberg, J. (登録日: 2013-09-05, 公開日: 2014-01-08, 最終更新日: 2023-12-20)

• 主引用文献: Knott, B.C.,Haddad Momeni, M.,Crowley, M.F.,Mackenzie, L.F.,Gotz, A.W.,Sandgren, M.,Withers, S.G.,Stahlberg, J.,Beckham, G.T.; The Mechanism of Cellulose Hydrolysis by a Two-Step, Retaining Cellobiohydrolase Elucidated by Structural and Transition Path Sampling Studies.; J.Am.Chem.Soc., 136:321-, 2014; PubMed: 24341799; DOI: 10.1021/JA410291U

実験手法

X-RAY DIFFRACTION (1.32 Å)