4C1S

Glycoside hydrolase family 76 (mannosidase) Bt3792 from Bacteroides thetaiotaomicron VPI-5482

4C1S の概要

• エントリーDOI: 10.2210/pdb4c1s/pdb
• 関連するPDBエントリー: 4C1R
• 分子名称: GLYCOSIDE HYDROLASE FAMILY 76 MANNOSIDASE, 1,2-ETHANEDIOL, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: hydrolase, gut microbiota
• 由来する生物種: BACTEROIDES THETAIOTAOMICRON VPI-5482
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87852.25

構造登録者

Cuskin, F.,Lowe, E.C.,Zhu, Y.,Temple, M.,Thompson, A.J.,Cartmell, A.,Piens, K.,Bracke, D.,Vervecken, W.,Munoz-Munoz, J.L.,Suits, M.D.L.,Boraston, A.B.,Williams, S.J.,Davies, G.J.,Abbott, W.D.,Martens, E.C.,Gilbert, H.J. (登録日: 2013-08-13, 公開日: 2013-11-13, 最終更新日: 2023-12-20)

主引用文献

Cuskin, F.,Lowe, E.C.,Temple, M.J.,Zhu, Y.,Cameron, E.A.,Pudlo, N.A.,Porter, N.T.,Urs, K.,Thompson, A.J.,Cartmell, A.,Rogowski, A.,Hamilton, B.S.,Chen, R.,Tolbert, T.J.,Piens, K.,Bracke, D.,Vervecken, W.,Hakki, Z.,Speciale, G.,Munoz-Munoz, J.L.,Day, A.,Pena, M.J.,Mclean, R.,Suits, M.D.,Boraston, A.B.,Atherly, T.,Ziemer, C.J.,Williams, S.J.,Davies, G.J.,Abbott, D.W.,Martens, E.C.,Gilbert, H.J.
Human Gut Bacteroidetes Can Utilize Yeast Mannan Through a Selfish Mechanism.
Nature, 517:165-, 2015

PubMed Abstract: Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a 'selfish' model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.

PubMed: 25567280
DOI: 10.1038/NATURE13995

実験手法

X-RAY DIFFRACTION (2.1 Å)