4C0Y

Cryo-EM reconstruction of empty enterovirus 71 in complex with a neutralizing antibody E18

4C0Y の概要

• エントリーDOI: 10.2210/pdb4c0y/pdb
• 関連するPDBエントリー: 4C0U 4C10
• EMDBエントリー: 2434
• 分子名称: VP1, VP2, VP3, ... (5 entities in total)
• 機能のキーワード: virus-immune system complex, ev71, antibody, e18, neutralizing, pathogen, virus/immune system
• 由来する生物種: HUMAN ENTEROVIRUS 71; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 124384.11

構造登録者

Plevka, P.,Perera, R.,Cardosa, J.,Suksatu, A.,Kuhn, R.J.,Rossmann, M.G. (登録日: 2013-08-08, 公開日: 2014-02-05, 最終更新日: 2024-05-08)

主引用文献

Plevka, P.,Lim, P.,Perera, R.,Cardosa, J.,Suksatu, A.,Kuhn, R.J.,Rossmann, M.G.
Neutralizing Antibodies Can Initiate Genome Release from Human Enterovirus 71.
Proc.Natl.Acad.Sci.USA, 111:2134-, 2014

PubMed Abstract: Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to "A" particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 °C and 37 °C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles.

PubMed: 24469789
DOI: 10.1073/PNAS.1320624111

実験手法

ELECTRON MICROSCOPY (16 Å)