4BZI

The structure of the COPII coat assembled on membranes

4BZI の概要

• エントリーDOI: 10.2210/pdb4bzi/pdb
• 関連するPDBエントリー: 4BZJ 4BZK
• EMDBエントリー: 2428 2430 2431
• 分子名称: SEC23P, SAR1P, SEC24P, ... (7 entities in total)
• 機能のキーワード: transport protein, secretion, trafficking, sec23, sec24, sar1
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 945064.64

構造登録者

Zanetti, G.,Prinz, S.,Daum, S.,Meister, A.,Schekman, R.,Bacia, K.,Briggs, J.A.G. (登録日: 2013-07-26, 公開日: 2013-09-18, 最終更新日: 2024-05-08)

主引用文献

Zanetti, G.,Prinz, S.,Daum, S.,Meister, A.,Schekman, R.,Bacia, K.,Briggs, J.A.G.
The Structure of the Copii Transport-Vesicle Coat Assembled on Membranes
Elife, 2:00951-, 2013

PubMed Abstract: Coat protein complex II (COPII) mediates formation of the membrane vesicles that export newly synthesised proteins from the endoplasmic reticulum. The inner COPII proteins bind to cargo and membrane, linking them to the outer COPII components that form a cage around the vesicle. Regulated flexibility in coat architecture is essential for transport of a variety of differently sized cargoes, but structural data on the assembled coat has not been available. We have used cryo-electron tomography and subtomogram averaging to determine the structure of the complete, membrane-assembled COPII coat. We describe a novel arrangement of the outer coat and find that the inner coat can assemble into regular lattices. The data reveal how coat subunits interact with one another and with the membrane, suggesting how coordinated assembly of inner and outer coats can mediate and regulate packaging of vesicles ranging from small spheres to large tubular carriers. DOI:http://dx.doi.org/10.7554/eLife.00951.001.

PubMed: 24062940
DOI: 10.7554/ELIFE.00951

実験手法

ELECTRON MICROSCOPY (23 Å)