4BY2

SAS-4 (dCPAP) TCP domain in complex with a Proline Rich Motif of Ana2 (dSTIL) of Drosophila Melanogaster

Summary for 4BY2

• Entry DOI: 10.2210/pdb4by2/pdb
• Descriptor: ANASTRAL SPINDLE 2, SAS 4, 1,2-ETHANEDIOL (3 entities in total)
• Functional Keywords: structural protein, cpap, stil, microcephaly, mcph, centriole, tcp domain, extended beta sheet
• Biological source: DROSOPHILA MELANOGASTER (FRUIT FLY); More
• Total number of polymer chains: 3
• Total formula weight: 85880.26

Authors

Cottee, M.A.,Muschalik, N.,Wong, Y.L.,Johnson, C.M.,Johnson, S.,Andreeva, A.,Oegema, K.,Lea, S.M.,Raff, J.W.,van Breugel, M. (deposition date: 2013-07-17, release date: 2013-09-18, Last modification date: 2024-05-08)

Primary citation

Cottee, M.A.,Muschalik, N.,Wong, Y.L.,Johnson, C.M.,Johnson, S.,Andreeva, A.,Oegema, K.,Lea, S.M.,Raff, J.W.,van Breugel, M.
Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly.
Elife, 2:e01071-e01071, 2013

PubMed Abstract: Centrioles organise centrosomes and template cilia and flagella. Several centriole and centrosome proteins have been linked to microcephaly (MCPH), a neuro-developmental disease associated with small brain size. CPAP (MCPH6) and STIL (MCPH7) are required for centriole assembly, but it is unclear how mutations in them lead to microcephaly. We show that the TCP domain of CPAP constitutes a novel proline recognition domain that forms a 1:1 complex with a short, highly conserved target motif in STIL. Crystal structures of this complex reveal an unusual, all-β structure adopted by the TCP domain and explain how a microcephaly mutation in CPAP compromises complex formation. Through point mutations, we demonstrate that complex formation is essential for centriole duplication in vivo. Our studies provide the first structural insight into how the malfunction of centriole proteins results in human disease and also reveal that the CPAP-STIL interaction constitutes a conserved key step in centriole biogenesis. DOI:http://dx.doi.org/10.7554/eLife.01071.001.

PubMed: 24052813
DOI: 10.7554/eLife.01071

Experimental method

X-RAY DIFFRACTION (2.57 Å)