4BXD

CRYSTAL STRUCTURE OF AMPDH3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH TETRASACCHARIDE PENTAPEPTIDE

4BXD の概要

• エントリーDOI: 10.2210/pdb4bxd/pdb
• 関連するPDBエントリー: 4BXE 4BXJ
• 分子名称: AMPDH3, PEPTIDE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-methyl 2-acetamido-2-deoxy-beta-D-glucopyranoside, ... (5 entities in total)
• 機能のキーワード: hydrolase-peptide complex, hydrolase/peptide
• 由来する生物種: PSEUDOMONAS AERUGINOSA PAO1; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 59851.49

構造登録者

Artola-Recolons, C.,Hermoso, J.A. (登録日: 2013-07-10, 公開日: 2013-10-09, 最終更新日: 2024-10-16)

主引用文献

Lee, M.,Artola-Recolons, C.,Carrasco-Lopez, C.,Martinez-Caballero, S.,Hesek, D.,Spink, E.,Lastochkin, E.,Zhang, W.,Hellman, L.M.,Boggess, B.,Hermoso, J.A.,Mobashery, S.
Cell-Wall Remodeling by the Zinc-Protease Ampdh3 from Pseudomonas Aeruginosa.
J.Am.Chem.Soc., 135:12604-, 2013

PubMed Abstract: Bacterial cell wall is a polymer of considerable complexity that is in constant equilibrium between synthesis and recycling. AmpDh3 is a periplasmic zinc protease of Pseudomonas aeruginosa , which is intimately involved in cell-wall remodeling. We document the hydrolytic reactions that this enzyme performs on the cell wall. The process removes the peptide stems from the peptidoglycan, the major constituent of the cell wall. We document that the majority of the reactions of this enzyme takes place on the polymeric insoluble portion of the cell wall, as opposed to the fraction that is released from it. We show that AmpDh3 is tetrameric both in crystals and in solution. Based on the X-ray structures of the enzyme in complex with two synthetic cell-wall-based ligands, we present for the first time a model for a multivalent anchoring of AmpDh3 onto the cell wall, which lends itself to its processive remodeling.

PubMed: 23931161
DOI: 10.1021/JA407445X

実験手法

X-RAY DIFFRACTION (3.1 Å)