4BWP

Structure of Drosophila Melanogaster PAN3 pseudokinase

4BWP の概要

• エントリーDOI: 10.2210/pdb4bwp/pdb
• 関連するPDBエントリー: 4BWK 4BWX
• 分子名称: PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUNIT PAN-3, AMP PHOSPHORAMIDATE (2 entities in total)
• 機能のキーワード: gene regulation, deadenylase, pan2, mirna, mrna decay
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 102830.82

構造登録者

Christie, M.,Boland, A.,Huntzinger, E.,Weichenrieder, O.,Izaurralde, E. (登録日: 2013-07-03, 公開日: 2013-08-21, 最終更新日: 2023-12-20)

主引用文献

Christie, M.,Boland, A.,Huntzinger, E.,Weichenrieder, O.,Izaurralde, E.
Structure of the Pan3 Pseudokinase Reveals the Basis for Interactions with the Pan2 Deadenylase and the Gw182 Proteins
Mol.Cell, 51:360-, 2013

PubMed Abstract: The PAN2-PAN3 deadenylase complex functions in general and miRNA-mediated mRNA degradation and is specifically recruited to miRNA targets by GW182/TNRC6 proteins. We describe the PAN3 adaptor protein crystal structure that, unexpectedly, forms intertwined and asymmetric homodimers. Dimerization is mediated by a coiled coil that links an N-terminal pseudokinase to a C-terminal knob domain. The PAN3 pseudokinase binds ATP, and this function is required for mRNA degradation in vivo. We further identified conserved surfaces required for mRNA degradation, including the binding surface for the PAN2 deadenylase on the knob domain. The most remarkable structural feature is the presence of a tryptophan-binding pocket at the dimer interface, which mediates binding to TNRC6C in human cells. Together, our data reveal the structural basis for the interaction of PAN3 with PAN2 and the recruitment of the PAN2-PAN3 complex to miRNA targets by TNRC6 proteins.

PubMed: 23932717
DOI: 10.1016/J.MOLCEL.2013.07.011

実験手法

X-RAY DIFFRACTION (3.6 Å)