4BWE

Crystal structure of C-terminally truncated glypican-1 after controlled dehydration to 86 percent relative humidity

4BWE の概要

• エントリーDOI: 10.2210/pdb4bwe/pdb
• 分子名称: Glypican-1, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: membrane protein, proteoglycan, glycosaminoglycans, heparan sulfate, glycoprotein, helical bundle
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 216104.95

構造登録者

Awad, W.,Svensson Birkedal, G.,Thunnissen, M.M.G.M.,Mani, K.,Logan, D.T. (登録日: 2013-07-01, 公開日: 2013-12-18, 最終更新日: 2024-11-20)

主引用文献

Awad, W.,Svensson Birkedal, G.,Thunnissen, M.M.,Mani, K.,Logan, D.T.
Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.
Acta Crystallogr. D Biol. Crystallogr., 69:2524-2533, 2013

PubMed Abstract: The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time Tinc. Of these, the most important was shown to be Tinc. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.

PubMed: 24311593
DOI: 10.1107/S0907444913025250

実験手法

X-RAY DIFFRACTION (2.46 Å)