4BNE

Pacsin2 Interacts with Membranes and Actin-Filaments

4BNE の概要

• エントリーDOI: 10.2210/pdb4bne/pdb
• 分子名称: PROTEIN KINASE C AND CASEIN KINASE SUBSTRATE IN NEURONS PROTEIN 2, SULFATE ION, TRIETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: endocytosis, membrane sculpting, f- actin binding
• 由来する生物種: GALLUS GALLUS (CHICKEN)
• 細胞内の位置: Cell junction, focal adhesion : O13154
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 106784.23

構造登録者

Kostan, J.,Salzer, U.,Orlova, A.,Toeroe, I.,Hodnik, V.,Schreiner, C.,Merilainen, J.,Nikki, M.,Virtanen, I.,Lehto, V.-P.,Anderluh, G.,Egelman, E.H.,Djinovic-Carugo, K. (登録日: 2013-05-15, 公開日: 2014-05-14, 最終更新日: 2024-05-08)

主引用文献

Kostan, J.,Salzer, U.,Orlova, A.,Toro, I.,Hodnik, V.,Senju, Y.,Zou, J.,Schreiner, C.,Steiner, J.,Merilainen, J.,Nikki, M.,Virtanen, I.,Carugo, O.,Rappsilber, J.,Lappalainen, P.,Lehto, V.,Anderluh, G.,Egelman, E.H.,Djinovic-Carugo, K.
Direct Interaction of Actin Filaments with F-Bar Protein Pacsin2.
Embo Rep., 15:1154-, 2014

PubMed Abstract: Two mechanisms have emerged as major regulators of membrane shape: BAR domain-containing proteins, which induce invaginations and protrusions, and nuclear promoting factors, which cause generation of branched actin filaments that exert mechanical forces on membranes. While a large body of information exists on interactions of BAR proteins with membranes and regulatory proteins of the cytoskeleton, little is known about connections between these two processes. Here, we show that the F-BAR domain protein pacsin2 is able to associate with actin filaments using the same concave surface employed to bind to membranes, while some other tested N-BAR and F-BAR proteins (endophilin, CIP4 and FCHO2) do not associate with actin. This finding reveals a new level of complexity in membrane remodeling processes.

PubMed: 25216944
DOI: 10.15252/EMBR.201439267

実験手法

X-RAY DIFFRACTION (2.57 Å)