4BMX

Native structure of futalosine hydrolase of Helicobacter pylori strain 26695

Summary for 4BMX

• Entry DOI: 10.2210/pdb4bmx/pdb
• Related: 4BMY 4BMZ 4BN0
• Descriptor: MTA/SAH NUCLEOSIDASE, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ADENINE, ... (4 entities in total)
• Functional Keywords: hydrolase
• Biological source: HELICOBACTER PYLORI
• Total number of polymer chains: 2
• Total formula weight: 55194.24

Authors

Kim, R.Q.,Offen, W.A.,Stubbs, K.A.,Davies, G.J. (deposition date: 2013-05-12, release date: 2013-09-11, Last modification date: 2023-12-20)

Primary citation

Kim, R.Q.,Offen, W.A.,Davies, G.J.,Stubbs, K.A.
Structural Enzymology of Helicobacter Pylori Methylthioadenosine Nucleosidase in the Futalosine Pathway
Acta Crystallogr.,Sect.D, 70:177-, 2014

PubMed Abstract: The recently discovered futalosine pathway is a promising target for the development of new antibiotics. The enzymes involved in this pathway are crucial for the biosynthesis of the essential prokaryotic respiratory compound menaquinone, and as the pathway is limited to few bacterial species such as the gastric pathogen Helicobacter pylori it is a potential target for specific antibiotics. In this report, the crystal structure of an H. pylori methylthioadenosine nucleosidase (MTAN; an enzyme with broad specificity and activity towards 6-amino-6-deoxyfutalosine), which is involved in the second step of menaquinone biosynthesis, has been elucidated at a resolution of 1.76 Å and refined with R factors of Rwork = 17% and Rfree = 21%. Activity studies on the wild type and active-site mutants show that the hydrolysis of 6-amino-6-deoxyfutalosine follows a mechanism similar to that of Escherichia coli MTAN. Further evidence for this mode of action is supplied by the crystal structures of active-site mutants. Through the use of reaction intermediates, the structures give additional evidence for the previously proposed nucleosidase mechanism. These structures and the confirmed reaction mechanism will provide a structural basis for the design of new inhibitors targeting the futalosine pathway.

PubMed: 24419390
DOI: 10.1107/S1399004713026655

Experimental method

X-RAY DIFFRACTION (1.76 Å)